Invitrogen™ Amplex™ Red/UltraRed Stop Reagent 

Description

Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. enic substrates and assay kits. It is designed to terminate reactions containing up to 0.1 units/mL of horseradish peroxidase (HRP) and 5μM hyrogen peroxide. After addition of the stop reagent, the fluorescence signal remains stable for at least 3 hours.

• Sensitive biomolecular assays based on hydrogen peroxide–generating enzyme systems linked to peroxidase–mediated oxidation of the fluorogenic Amplex UltraRed or Amplex Red substrates
• Detection reactions are performed in microplate wells and are initiated by adding fluorogenic Amplex UltraRed or Amplex Red substrate, resulting in a continuous fluorescence increase that proceeds for 30 minutes or more
• Unknown analyte concentrations are determined by comparing fluorescence intensities measured at a certain time point during the reaction to parallel measurements at same time point on standard samples of known concentration
• It is critical to ensure that timing of standard and unknown sample measurements is same

Build Your Own Immunoassay, Cell Analysis, Enzyme and Protein Activity Assays, Immunoassays, Lipase and Phopholipase Activity, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Ready-To-Use Immunoassay

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