Detect activity levels as low as 0.002U/mL in one hour
Supplier: Invitrogen™ A12217
The Amplex Red Acetylcholine/Acetylcholinesterase Assay Kit provides an ultrasensitive method for detecting acetylcholinesterase (AChE) activity in a fluorescence microplate reader or fluorometer. AChE activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red reagent), a sensitive fluorogenic probe for hydrogen peroxide. AChE converts the acetylcholine substrate to choline, which is then oxidized by choline oxidase to betaine and hydrogen peroxide. In the presence of horseradish peroxidase, hydrogen peroxide reacts with the Amplex Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin.
Drug Discovery and Development, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation
Shipping Condition: Room temperature
| Store in freezer -5°C to -30°C and protect from light. | |
| Acetylcholine/Acetlycholinesterase Assay | |
| Acetylcholine⁄Acetlycholinesterase Assay | |
| 500 assays | |
| Molecular Substrate | |
| Acetylcholinesterase |
| Fluorescence Intensity | |
| Fluorescence Microplate Reader | |
| Amplex | |
| Room Temperature | |
| HRP (Horseradish Peroxidase) Substrate, Acetylcholinesterase Substrate |
For Research Use Only. Not for use in diagnostic procedures.
