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Invitrogen™ Human MMP3 Matched Antibody Pair

Catalog No. CHC1543
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10 x 96 Tests
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CHC1543 10 x 96 Tests
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Catalog No. CHC1543 Supplier Invitrogen™ Supplier No. CHC1543

Description

ELISA

The Human MMP3 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MMP3. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form, and is activated by extracellular proteinase cleavage. MMP3 is capable of binding to zinc and calcium ions, and has metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin. It is involved in normal cellular processes such as wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.

Specifications

Accession Number P08254
Assay Range 1.25-20 ng/mL
Assay Sensitivity <1.25 ng/mL
Conjugate HRP
Gene Accession No. P08254
Gene Alias CHDS6; EC 3.4.24.17; EMS-2; matrix metallo protease; matrix metallopeptidase 3; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3; matrix metalloproteinase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 precursor; matrix metalloproteinase-3; metalloproteinase 3 receptor; MMP; MMP10; MMP3; MMP-3; MMP-3 protein; MMPs; progelatinase; proteoglycanase; SL-1; SLN1; SLN-1; STMY; STMY1; Str1; STR-1; stromelysin; stromelysin 1; Stromelysin1; stromelysin-1; Transin1; Transin-1CHDS6; EC 3.4.24.17; EMS-2; matrix metallo protease; matrix metallopeptidase 3; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3; matrix metalloproteinase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 precursor; matrix metalloproteinase-3; metalloproteinase 3 receptor; MMP; MMP10; MMP3; MMP-3; MMP-3 protein; MMPs; progelatinase; proteoglycanase; SL-1; SLN1; SLN-1; STMY; STMY1; Str1; STR-1; stromelysin; stromelysin 1; Stromelysin1; stromelysin-1; Transin1; Transin-1; CHDS6; MMP-3; SL-1; STMY; STMY1; STR1
Gene Symbols MMP3
Immunoassay Kit Format Matched Antibody Pair
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Quantity 10 x 96 Tests
Regulatory Status RUO
Substrate Type Proteases
Target Species Human
Gene ID (Entrez) 4314
For Use With (Equipment) Absorbance Microplate Reader
Includes Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Content And Storage Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gene Symbol MMP3
Kit Contents Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Label or Dye Biotin
Protein Aliases SL-1, Matrix metalloproteinase-3, MMP-3, Transin-1
Shipping Condition Wet Ice
Storage Requirements 2-8°C
Target MMP3
Test Time 1 hr 30 min
Total Assay Time 24 hr
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