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Applied Biosystems™ MiniAmp™ Thermal Cycler
Description
The Applied Biosystems MiniAmp Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab's basic thermal cycling needs. The MiniAmp Thermal Cycler has a 5-inch color touchscreen, making it easy to control, and it can also be accessed through Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer.
Key features and benefits:
- Small footprint—With dimensions of 19 cm wide and 39 cm deep, you can fit the MiniAmp Thermal Cycler almost anywhere. And because its airflow is front to back, you can set multiple units side by side to save valuable bench space.
- 5-inch intuitive color touchscreen—for easy programming and training of new users
- WiFi-enabled and accessible by Thermo Fisher Connect—giving you the freedom to design and securely upload your methods from any mobile device or desktop computer
Applied Biosystems PCR plastics
Applied Biosystems PCR plastics have been designed and validated to work with our thermal cyclers for more than 25 years. Options for every format and throughput need are available to ensure consistent, precise results for any experiment. To find the best match please visit our Plastics Selection Guide.
Specifications
Specifications
| Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
| Content And Storage | • MiniAmp Thermal Cycler • Country-specific power cord |
| High-throughput Compatibility | High-throughput Compatible |
| For Use With (Equipment) | Applied Biosystems GeneAmp plastics consumables |
| Thermal Uniformity | <0.5°C (20 sec. after reaching 95°C) |
| Reaction Speed | Fast, Standard |
| Dimensions | 15.4 x 7.5 x 7.9 in. (39 x 19 x 20 cm) |
| Capacity | 96-well, 0.2 mL |
| Voltage | 100/240 V |
| Display Type | 5 in. Color TFT LCD Touchscreen |
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Frequently Asked Questions (FAQs)
The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:
Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).
There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.
As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.
Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf
There are 2 key differences: - The MiniAmp Plus Thermal Cycler has 3 VeriFlex zones while the MiniAmp Thermal Cycler does not. - The MiniAmp Plus Thermal Cycler has a block ramp rate of 3.5 degrees C/sec while the MiniAmp Thermal Cycler has a block ramp rate of 3.0 degrees C/sec.
Like the ProFlex PCR System and SimpliAmp Thermal Cycler, the MiniAmp/MiniAmpPlus Thermal Cycler can be connected wirelessly with an optional High-Power USB W-Fi Module, Cat. No. A26774.
To add an infinite hold, select the infinity symbol for program.
For Research Use Only. Not for use in diagnostic procedures.