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Applied Biosystems™ MicroSEQ™ 500 16S rDNA Sequencing Kit DFS Item

The sequencing component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria.

Supplier:  Applied Biosystems™ 4346480

Catalog No. 50-591-785


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Description

Description

  • The sequencing component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria
  • Use this kit to sequence PCR products that have been generated using the MicroSEQ™ 500 16S rDNA PCR Kit
  • The two kits together permit the quick and convenient amplification and sequencing of 500 base pairs of the 16S rRNA gene
  • Sequences can then be compared to the MicroSEQ™ 16S rDNA 500 Library to generate an identity match
  • This kit does not include a protocol or a Quick Reference Card
  • For Research Use Only. Not for use in diagnostics procedures.
  • Store at -15° to -25°C

Benefits of the MicroSEQ™ 500 16S rDNA Sequencing Kit

  • Sequence-based measurement ensures you get reproducible results in under 24 hours
  • Growth requirement-independent measurement enables you to identify bacteria using objective criteria
  • Universal domain-level primers eliminate up-front optimization
  • MicroSEQ™ ID Analysis Software ensures accurate species identification
  • Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms
  • Requires minimal training-same work flow as the MicroSEQ™ D2 LSU fungal kit
Specifications

Specifications

Each kit contains:
Two tubes each of forward and reverse sequencing mix. Sufficient reagent to perform 55 forward and 55 reverse sequencing reactions. Store at -15 to -25°C.
Kit
310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
Forward sequencing mix (2 tubes), and reverse sequencing mix (2 tubes)
55 Forward, 55 Reverse
Sequencing Kit
MicroSEQ™ ID Analysis Software
1st 500 bp of 16S rRNA gene
SBT (Sequence-Based Typing)
Capillary Electrophoresis
PCR Products
Sequencing
Customizable, Bacterial Library (> 2000 species)
MicroSEQ™
1 Kit
Dry Ice
Bacteria
Cycle Sequencing
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For Research Use Only. Not for use in diagnostic procedures.