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  6. Invitrogen™ MHC Class II (I-Ek) Monoclonal Antibody (14-4-4S), eBioscience™, Invitrogen™

Invitrogen™ MHC Class II (I-Ek) Monoclonal Antibody (14-4-4S), eBioscience™, Invitrogen™

Catalog No. 5013394
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5013394 50 μg
5013395 100 μg
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Catalog No. 5013394 Supplier Invitrogen™ Supplier No. 14598081

Description

Mouse Monoclonal Antibody

Description: The 14-4-4S monoclonal antibody reacts with the mouse and rat major histocompatibility complex class II, I-E subregion-encoded glycoprotein in mice strains of H-2^k, H-2^d, H-2^p and H-2^r. H-2^b, H-2^f and H-2^s haplotypes do not express the I-E antigen. MHC class II is present on B cells, monocytes, macrophages, and dendritic cells. The 14-4-4S mAb has been reported to block antigen presentation in in vitro assays. This antibody cross-reacts with rat. Applications Reported: 14-4-4S has been reported for use in flow cytometric analysis, immunoprecipitation, complement-dependent cytotoxity and immunohistochemical staining of frozen sections (Biotinylated 14-4-4S, cat. 13-5980, is recommended for IHC). Applications Tested: The 14-4-4S antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

Antigen MHC Class II (I-Ek)
Applications Flow Cytometry
Classification Monoclonal
Clone 14-4-4S
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene H2-Eb1
Gene Accession No. P04230, P14439
Gene Alias AI323765; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-IE-alpha; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia3; Ia-3; Ia4; Ia-4; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; major histocompatibility complex H-2E beta chain; MGC117888; MHC class II antigen E alpha; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein
Gene Symbols H2-Ea-ps, H2-Eb1
Host Species Mouse
Purification Method Affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 100504404, 14969
Target Species Mouse, Rat
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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For Research Use Only.