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Thermo Scientific™ Melon™ Gel Purification Buffer

Catalog No. PI89972
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1 pack for 200 mL
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PI89972 1 pack for 200 mL
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Catalog No. PI89972 Supplier Thermo Scientific™ Supplier No. 89972
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Description

Rapidly purify human, mouse and rabbit IgG from serum and other sources with this simpler alternative to traditional Protein A and Protein G methods.

Thermo Scientific™ Melon Gel IgG Purification Resins and Kits comprise a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.

Melon Gel IgG Purification Kits enable fast, easy and gentle purification of antibodies from serum and other sample types. Antibody purification with Melon Gel may be performed with gravity-flow columns, spin columns or chromatography cartridges. Melon Gel IgG Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody. Compared to antibody affinity purification with Protein A or Protein G, Melon Gel IgG Purification is simpler, faster and provides greater yields and higher purity for a wider variety of antibodies species.

Highlights:

  • Simple, one-step protocol – IgG Purification with Melon Gel Resin does not require the binding, washing, and multiple elution steps required by Protein A and Protein G

  • Rapid purification – antibody purification from serum is four to six times faster than Protein A or G methods

  • High recovery and purity – antibody recovery from serum for many species is greater than 90% with greater than 80% purity

  • Minimal dilution – reduced need to concentrate antibody before storage or use

  • Robust purification – Melon Gel IgG Purification Kits work with a wide range of antibodies including many that do not purify well on Protein A or Protein G

  • Gentle purification – no harsh antibody elution conditions helps retain IgG activity

  • Reusable support – Melon Gel Resin can be used for multiple antibody purifications

  • Available in various formats – spin columns, purification kits and chromatography cartridges

Specifications

Product Type Gel Purification Buffer
Product Line Melon
Reagent Type Binding Buffer
Description Melon Gel Purification Buffer
Content And Storage 20°C to 25°C
Quantity 1 pack for 200 mL
Form Powder

Frequently Asked Questions (FAQs)

Do you offer a kit for antibody purification?

For purification of antibodies, we offer Melon Gel, which binds other proteins from serum, ascites or culture supernatant, allowing the IgG to be collected in the flow-through. As low pH is typically used to elute IgG from Protein A, G, A/G or L, the use of Melon Gel can spare the IgG from exposure to such harsh conditions, which can be particularly important for monoclonal antibodies.

For Research Use Only. Not for use in diagnostic procedures.