missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Invitrogen™ MEGAscript™ RNAi Kit with Manual DFS Item

MEGAscript™ RNAi Kit with Manual

Manufacturer:  Invitrogen™ AM1626M

Catalog No. AM1626M



Description

Description

The Invitrogen™ MEGAscript™ RNAi Kit is for the synthesis of large mass amounts of dsRNA for RNAi experiments in nonmammalian systems. The MEGAscript RNAi Kit uses Invitrogen's patented high-yield transcription technology and includes reagents for transcription, nuclease digestion, and clean up. The kit includes sufficient reagents for 20 reactions.

Synthesize Large Amounts of dsRNA:
The ability to generate large amounts of clean, double-stranded RNA is essential for the success of RNAi experiments. The MEGAscript RNAi Kit allows RNAi researchers to synthesize 50 to 100μg or more of double-stranded RNA in a single transcription reaction. The yield is typically 10–50 times the amount of RNA produced by conventional transcription reactions. DNA of either PCR or plasmid origin can be used as a template for the transcription reaction. The sense and antisense strands can be synthesized from a PCR-generated DNA template containing T7 RNA Polymerase promoters on both ends of the template, eliminating the need for a separate annealing step.
Specifications

Specifications

T7 Enzyme Mix, 10X T7 Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, 10X Binding Buffer, Elution Solution, RNase, DNase I, 10X Digestion Buffer, 2X Wash Solution, and Control Template are all stored at –20°C, Nuclease-free Water may be store at any temperature. Filter Cartridges and Collection Tubes are stored at room temperature.
Double-Stranded RNA (Long)
Ambion™, MEGAscript™
Transcription Control
20
Wet Ice
SDS
Documents

Documents

Product Certifications
Promotions

Promotions

For Research Use Only. Not for use in diagnostic procedures.