PeproTech MCP-4 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified by affinity chromatography employing an immobilized Human MCP-4 matrix. Sandwich ELISA: To detect Human MCP-4 (CCL13) by sandwich ELISA (using 100 μL/well) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MCP-4 (CCL13) (500-P04BT) as a detection antibody, allows the detection of at least 2000- 4000 pg/mL of Recombinant Human MCP-4 (CCL13). Western Blot: To detect Human MCP-4 (CCL13) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-4 (CCL13) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFalpha. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response.