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Invitrogen™ Multispecies MAPK Family Activation InstantOne™ ELISA Kit
Description
The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated ERK 1/2 (human, mouse, hamster), p38 MAPK (human, mouse), and JNK 1/2 (human, mouse) in cell lysates. Detection of these phosphorylated molecules in rat is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
MAP kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. MAPKBP-1 (mitogen-activated protein kinase binding protein 1), also known as JNKBP-1, is a 1,514 amino acid protein that contains twelve WD repeats. Induced by TRAF2 (TNF receptor-associated factor 2) and Tak1 (TGF-β-activated kinase 1), MAPKBP-1 is thought to act an adaptor protein for NFκB (nuclear factor κ-B) activation. MAPKBP-1 interacts with JNK3 and may promote TRAF2 polyubiquitination. MAPKBP-1 exists as six alternatively spliced variants and is encoded by a gene located on human chromosome 15. Human chromosome 15 houses over 700 genes and comprises nearly 3% of the human genome. Angelman syndrome, Prader-Willi syndrome, Tay-Sachs disease and Marfan syndrome are all associated with defects in chromosome 15-localized genes.
Specifications
Specifications
| Accession Number | P27361, P28482, Q16539 |
| Content And Storage | • User Manual • phospho-ERK 1/2 (Thr202/Tyr204, Thr182/Tyr187) Capture Antibody Reagent, 1 mL • phospho-ERK 1/2 (Thr202/Tyr204, Thr182/Tyr187) Detection Antibody Reagent, 1 mL • phospho-p38 MAPK (Thr180/Tyr182) Capture Antibody Reagent, 1 mL • phospho-p38 MAPK (Thr180/Tyr182) Detection Antibody Reagent, 1 mL • phospho-JNK 1/2 (Thr183/Tyr185) Capture Antibody Reagent, 1 mL • phospho-JNK 1/2 (Thr183/Tyr185) Detection Antibody Reagent, 1 mL • InstantOne Assay Plate, 96 well (sealed) • Cell Lysis Buffer (5X), 10 mL • Enhancer Solution, 1 mL • Wash Buffer (10X), 15 mL • Detection Reagent (TMB Substrate), 12 mL • Stop Solution, 12 mL • Positive Control Cell Lysate lyophilized, 0.25 mL • Plate seal, 1 ea |
| Conjugate | HRP |
| Immunoassay Kit Format | Coated ELISA Kit |
| Product Type | ELISA |
| Sample Type | Cell Lysates |
| Target Species | Human, Mouse |
| For Use With (Application) | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene Alias | MAPKMAPK; ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK;ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; p44-ERK1; p44-MAPK;JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; SAPK1c;CSBP; CSBP1; CSBP2; CSPB1; |
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