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Applied Biosystems™ MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS)
Description
- Kit is designed to recover viral RNA from dried blood spots (DBS) and plasma for HIV-1 drug resistance testing
- MagMAX magnetic-bead technology ensures reproducible recovery of high-quality HIV-1 viral RNA compatible with downstream applications such as RT-PCR and Sanger sequencing
- Automation: Workflows are designed for the KingFisher Flex and KingFisher Duo Prime systems
- Sensitivity: Extracts amplifiable viral RNA from DBS and plasma samples
- DBS: >2000 copies/mL
- Plasma: >1000 copies/mL
- Duration: Test takes less than 60 minutes: ∼30 min DBS lysis, ∼30 min viral RNA extraction
Benefits of MagMAX magnetic bead–based purification:
- Beads bind the nucleic acid more efficiently than glass-fiber filters, resulting in higher and more consistent yields
- Since filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples
- This clogging issue is of particular concern with protein-rich, large-volume samples such as whole blood or plasma that are commonly used for viral testing
Specifications
Specifications
| Bead Type | Magnetic |
| Content And Storage | • 60 mL Dried Blood Spots Lysis Solution • 55 mL Binding Solution • 100 mL Wash Buffer • 10 mL Elution Solution • 1 mL Proteinase K • 2 mL Total Nucleic Acid Binding Beads |
| Detection Method | HIV-1 viral RNA compatible with downstream applications such as RT-PCR and Sanger sequencing |
| Sample Type | Whole Blood, Plasma |
| For Use With (Equipment) | KingFisher Duo Prime, KingFisher Flex, Magnetic Stand |
| No. of Reactions | 100 reactions |
| Quantity | 100 reactions |
Frequently Asked Questions (FAQs)
The MagMAX Dried Blood Spots Lysis Solution is different from the Binding Solution in the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (Cat. No. A42352, A48310).
The approximate extraction workflow time with the KingFisher Flex instrument is <90 min for 24 DBS samples and <60 min for 24 plasma samples. With the KingFisher Duo instrument, the approximate extraction workflow time is <75 min for 12 DBS samples and <45 min for 12 plasma samples.
The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) was validated using 200 µL of plasma sample. However, the standard protocol mentioned in the product manual uses reagent volumes that can work with 200-400 µL of sample.
The DBS lysate volume is variable because the filter paper material absorbs anywhere from 150-250 µL of the starting 600 µL of Dried Blood Spots Lysis Solution during incubation. If two 13 mm diameter cutouts are used instead of one, the DBS lysate volume recovered may be even less.
The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit, for HIV-1 dried blood spots (DBS) (Cat. No. A53770) was validated using 1.5 mL non-stick tubes per 13 mm diameter DBS cutout. However, the 1.5 mL tubes have a narrow opening which requires folding the 13 mm DBS cutout into a “taco” shape prior to inserting into the tube. Other tubes (eg., Sarstedt 8 mL) have been used during development and work well, but have more dead space which may result in more sample evaporation.
For research use only. Not for use in diagnostic procedures.