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Applied Biosystems™ MagMAX™ Pathogen RNA/DNA Kit
Description
PCR inhibitors are effectively removed in the process, making the resulting nucleic acid ideal for real-time PCR and RT-PCR applications.
Key Features:
- Convenience and flexibility: one kit for RNA and DNA, suitable for a wide range of sample types sample input volumes
- Increased confidence in your results: fewer false-negatives due to effective PCR inhibitor removal
- Improved workflow efficiency: process 96 samples in approximately 45 minutes using the MagMAX™ Express-96 Deep Well Magnetic Particle Processor
The MagMAX™ Pathogen RNA/DNA Kit has been optimized to provide a convenient, flexible solution. This single kit can accomodate:
- Purification of RNA and DNA
- Purification from viruses and easy-to-lyse bacteria and parasites
- Low and high sample input volumes
- A wide range of sample types:
- Low cell content samples (e.g., serum and plasma; nasal, tracheal, and cloacal swabs; ear notches)
- Whole blood
- Semen
- Oral fluid
- Feces
- Biomed Diagnostics InPouch™ TF (Tritrichomonas foetus) culture
The MagMAX™ Pathogen RNA/DNA Kit is Easy to Automate
Purify RNA/DNA from 96 samples in approximately 45 minutes using the MagMAX™ Express-96 Deep Well Magnetic Particle Processor. Low and high sample input volumes can be used for most sample types, and multiple sample types can be processed at the same time on the same plate after sample-specific pre-processing, saving time and plastics.
Increase the Accuracy of Your Results
Reduce false-negative results due to the effective PCR inhibitor removal achieved by the MagMAX™ Pathogen RNA/DNA Kit. Magnetic particles bind RNA/DNA more efficiently and are more thoroughly washed than glass fiber filters, resulting in higher yields and purity of RNA/DNA. In addition, no sample cross contamination has been observed due to extensive optimization of the MagMAX™ Express-96 Deep Well Magnetic Particle Processor programs.
Specifications
Specifications
| Content And Storage | Store at room temperature: 1 bottle of Lysis⁄Binding Solution Concentrate 1 bottle of Wash Solution 1 Concentrate 2 bottles of Wash Solution 2 Concentrate 1 bottle of Elution Buffer Store at 4 °C: 1 bottle of Nucleic Acid Binding Beads Store at -20 °C: 1 bottle of Lysis ENHANCER 5 tubes of Carrier Nucleic Acid |
| Format | 96-well Plate |
| Isolation Technology | Magnetic Bead |
| Purification Time | 45 min. |
| Sample Type | Any Swab Type, Bacteria, BioMed Diagnostics InPouch™ TF Culture, Cell-free Biological Fluids, Ear Notches, Feces, Mosquito, Parasite Samples, Plasma, Saliva, Serum, Tissue Homogenates, Viral Samples, Viral Transport Media Samples, Whole Blood |
| Final Product Type | Viral DNA⁄RNA, Pathogen DNA⁄RNA |
| High-throughput Compatibility | High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols |
| For Use With (Equipment) | KingFisher™ Flex Magnetic Particle Processor, MagMAX™ Express-96 Deep Well Magnetic Particle Processor, MagMAX™ Express-96 Standard Magnetic Particle Processor, KingFisher 96, MagMAX™ Express Magnetic Particle Processor |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), PCR |
| No. of Reactions | 480 preps |
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Frequently Asked Questions (FAQs)
The MagMAX Pathogen RNA/DNA Kit is designed for rapid purification of nucleic acid (RNA and DNA) from the following sample types:
- Low-cell content samples including serum, plasma, swabs (nasal, tracheal, and cloacal), ear notches
- Whole blood
- Semen
- Oral fluid
- Feces
- Biomed Diagnostics InPouch TF (Tritrichomonas foetus) culture
The manual isolation protocol for the MagMax Pathogen RNA/DNA Kit can be found in the MagMAX Pathogen RNA/DNA Kit Protocol , page 49.
We recommend using DNA Binding Beads for MagMAX-96 DNA Multi-Sample Kit (Cat. No. 4489112) with the MagMax Pathogen RNA/DNA Kit.
No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.
For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.