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  5. Thermo Scientific™ MabCaptureC™ High Capacity Protein A Resin

Thermo Scientific™ MabCaptureC™ High Capacity Protein A Resin

Catalog No. PIA53034
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PIA53034 100 mL
PIA53031 5 mL
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A40004564 2 Plates
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Catalog No. PIA53034 Supplier Thermo Scientific™ Supplier No. A53034
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Description

MabCaptureC High-Capacity Protein A Spin Columns provide a fast, ready-to-use, method for capturing monoclonal antibodies (mAbs) and recombinant Fc-tagged fusion proteins.

GoPure MabCaptureC Screening Plates, 96-well, 50 μL, contain MabCaptureC High-Capacity Protein A Resin, a high-performance chromatography resin for capturing monoclonal antibodies (mAbs) and recombinant Fc-tagged fusion proteins. It provides high specific-binding capacity and enhanced alkaline-stability characteristics under clean-in-place conditions (CIP), for more robust purification and resin regeneration. These ready-to-use, 96-well spin plates offer a high-throughput format for rapid antibody purification, screening, and evaluating multiple conditions in a single plate experiment.

Features of MabCaptureC High Capacity Protein A Resin

  • High binding capacity—60 mg hIgG/mL at 5-minite residence (80 mg/mL at 10-minute residence)
  • Alkaline stability—engineered to withstand common CIP procedures with 0.2 M NaOH, enabling efficient cleaning of the chromatography resin and improving product quality
  • Animal component-free raw materials
  • Versatility—clean antibody or Fc-fusion protein preparations from cell culture supernatant, serum, or ascites
  • Stability—high-affinity Protein A is securely immobilized to high purity agarose to prevent leaching of ligand, target antibody, or base support
  • Flexible— available in multiple formats, including bulk loose resin, spin columns, and 96-well spin plates

The Protein A ligand, which is produced in yeast, is immobilized on a rigid and uniformly sized agarose bead with an average particle size of 75 μm. The uniformity of the particle size, in turn, delivers superior performance characteristics over traditional resins.

MabCaptureC High-Capacity Protein A Resin, alkaline-stable, is suitable for the purification of mAbs as well as a subpopulation of scFv's and Fab fragments that contain a VH3 domain.

Available formats

  • Resin slurries—10 mL, 50 mL, and 200 mL of 50% slurry
  • Spin columns—10 mL spin columns containing 1 mL of settled resin, supplied at 5 mL 20% slurry
  • GoPure 96-well spin plates—96-well spin plates with 20 μL or 50 μL of settled resin

Specifications

Product Type High Capacity Protein A Resin
Column Type Agarose Resin, Affinity
Format Bottle
Stationary Phase Protein A
Binding Capacity 60 mg hIgG/mL at 5 min residence (80 mg/mL at 10 min residence)
Support Type Cross linked Agarose
Formulation 50% Slurry
Description High Capacity Protein A Resin, Alkaline Stable
Content And Storage 100 mL of resin provided as a 200 mL 50% slurry. Upon receipt store at 4°C.
Quantity 100 mL

Frequently Asked Questions (FAQs)

Can I automate the workflow using MabCaptureC High Capacity Protein A Resin?

Yes. FPLC (Fast protein liquid chromatography) use is outlined in the instruction manual, along with compatible buffer formulations, proper flow rates, and clean-in-place protocols.
Using MabCaptureC High Capacity Protein A Resin, what downstream assays are compatible with the eluted samples?

Purified samples eluted from MabCaptureC High Capacity Protein A Resin are compatible with protein assays (Bradford, BCA, Rapid Gold, A280, etc.) and can be used for industry standard testing applications (western blotting, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, etc.).
What is the maximum operational flow velocity that can be used with MabCaptureC High Capacity Protein A Resin?

The maximum operational flow velocity with MabCaptureC High Capacity Protein A Resin is 300 cm/hr or less (150 cm/hr recommended).
What is the pH stability of MabCaptureC High Capacity Protein A Resin?

MabCaptureC High Capacity Protein A Resin is stable at pH 2-14 (short-term storage) and pH 3-14 (long-term storage).
After regeneration of MabCaptureC High Capacity Protein A Resin, is there a specific buffer in which the beads should be stored?

If the beads are not going to be re-used immediately, we recommend using 20% ethanol for long-term storage.
How do I regenerate MabCaptureC High Capacity Protein A Resin?

Using the Clean-In-Place (CIP) protocol provided in the instruction manual, we recommend cleaning the MabCaptureC High Capacity Protein A Resin with 10 CV (column volumes) of 0.2 M NaOH after every run. The resin has limited stability at 0.5 M NaOH, but this can be used in an optimized cleaning strategy after every 10th or 20th cycle.
How many uses/regenerations are possible with MabCaptureC High Capacity Protein A Resin?

MabCaptureC High Capacity Protein A Resin can be regenerated and reused for over 100 times without affecting protein yield or purity (depending on the sample type).
What is the dynamic binding capacity of MabCaptureC High Capacity Protein A Resin?

The dynamic binding capacity of MabCaptureC High Capacity Protein A Resin is 60 mg hIgG/mL at 5 min residence time, going up to 80 mg hIgG/mL at 10 min residence time.
What is the particle size of the MabCaptureC High Capacity Protein A Resin?

The Protein A ligand, which is produced in yeast, is immobilized on a rigid and uniformly sized agarose bead with an average particle size of 75 µm.
What is the resin matrix in MabCaptureC High Capacity Protein A Resin?

MabCaptureC High Capacity Protein A Resin is composed of highly cross-linked agarose beads (6%).
Is MabCaptureC High Capacity Protein A Resin suited for bioprocessing?

Yes. The Alkaline-stable Protein A resin is compatible with many different formats for bioprocessing (serum, ascites, CHO cells, Expi293 cells, etc.).

For Research Use Only. Not for use in diagnostic procedures.