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MilliporeSigma™ LysargiNase

Numéro de catalogue. EMS0008
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Numéro de catalogue. Quantity
EMS0008 1/Ea.
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Numéro de catalogue. EMS0008 Fournisseur MilliporeSigma™ Code fournisseur EMS0008
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Description

MilliporeSigma™ LysargiNase is suitable for peptide or protein digestion in solution or in gel.

  • LysargiNase is a metalloproteinase found in the thermophilic archaea Methanosarcina acetivorans
  • It specifically cleaves before lysine and arginine residues in proteins
  • This cleavage results in peptides with molecular weights similar to tryptic peptides, but with N-terminal lysine or arginine residues that can be fragmented with b ion-dominated spectra
  • This can improve protein C-terminal peptide identification and several arginine rich phosphosite assignments
  • Unlike with trypsin, LysargiNase cleavage also occurs at methylated lysine and arginine, allowing detection of these epigenetic modifications
  • For peptide or protein digestion, a ratio of between 1:100 to 1:20 (w/w) enzyme:substrate is recommended
  • It is active up to 55°C at pH 6 to 9
  • LysargiNase is reversibly inhibited by 1, 10-phenanthroline, EDTA and other calcium and zinc chelating agents
  • It is active under a variety of solvent conditions including 5 mM TCEP, 5% methanol, 5% acetonitrile, 0.8 M urea, 0.1% RapiGest, 1% deoxycholate, 0.2% SDS and 1% NP-40

Spécifications

Description Mirrors trypsin for protein C-terminal and methylation-site identification
Quantity 1/Ea.
Kit Contents 20 μg LysargiNase, prepared by recombinant expression in E. coli and lyophilized from buffer
For Use With (Application) Suitable for peptide or protein digestion in solution or in gel
Content And Storage Store the lyophilized product at -20°C for up to 2 years. After reconstitution, the unused material can be stored in the freezer in aliquots. Avoid repeated freeze thaw cycles.