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MilliporeSigma™ LysargiNase
Description
- LysargiNase is a metalloproteinase found in the thermophilic archaea Methanosarcina acetivorans
- It specifically cleaves before lysine and arginine residues in proteins
- This cleavage results in peptides with molecular weights similar to tryptic peptides, but with N-terminal lysine or arginine residues that can be fragmented with b ion-dominated spectra
- This can improve protein C-terminal peptide identification and several arginine rich phosphosite assignments
- Unlike with trypsin, LysargiNase cleavage also occurs at methylated lysine and arginine, allowing detection of these epigenetic modifications
- For peptide or protein digestion, a ratio of between 1:100 to 1:20 (w/w) enzyme:substrate is recommended
- It is active up to 55°C at pH 6 to 9
- LysargiNase is reversibly inhibited by 1, 10-phenanthroline, EDTA and other calcium and zinc chelating agents
- It is active under a variety of solvent conditions including 5 mM TCEP, 5% methanol, 5% acetonitrile, 0.8 M urea, 0.1% RapiGest, 1% deoxycholate, 0.2% SDS and 1% NP-40
Specifications
Specifications
| Description | Mirrors trypsin for protein C-terminal and methylation-site identification |
| Quantity | 1/Ea. |
| Kit Contents | 20 μg LysargiNase, prepared by recombinant expression in E. coli and lyophilized from buffer |
| For Use With (Application) | Suitable for peptide or protein digestion in solution or in gel |
| Content And Storage | Store the lyophilized product at -20°C for up to 2 years. After reconstitution, the unused material can be stored in the freezer in aliquots. Avoid repeated freeze thaw cycles. |