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  6. Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Catalog No. A72127
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Quantity:
100 Reactions
120 μg
1600 μg
2000 μL
Product Type:
Lyo-ready RPA Kit
Lyo-ready T4 Gene 32 Protein
Lyo-ready T4 UvsX Protein
Lyo-ready T4 UvsY Protein
Reaction Buffer
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Catalog No. Quantity Product Type
A72127 100 Reactions Lyo-ready RPA Kit
A72123 1600 μg Lyo-ready T4 Gene 32 Protein
A72124 120 μg Lyo-ready T4 UvsX Protein
A72125 120 μg Lyo-ready T4 UvsY Protein
A72126 2000 μL Reaction Buffer
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Catalog No. A72127 Supplier Invitrogen™ Supplier No. A72127
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Description

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

  • Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
  • Robust and specific RNA/DNA amplification
  • Amplification from inhibitor-containing samples
  • Fast target amplification in as little as 20 minutes
  • Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

  • Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
  • Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
  • Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
  • Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
  • Optimized reaction buffer, magnesium chloride, and dNTPs

Specifications

Product Type Lyo-ready RPA Kit
Form Liquid
For Use With (Application) RPA, RT-RPA
Polymerase Lyo-ready Bst DNA Polymerase
Quantity 100 Reactions
Research Category Nucleic Acid Amplification
Content And Storage

• Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL
• Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL
• Lyo-ready Bst DNA Polymerase (6 U/μL), 50 μL
• Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL
• 2X RPA Reaction Buffer, 1000 μL
• 280 mM MgCl2, 100 μL
• 10 mM dNTP Mix, 50 μL

Store at -25°C to -15°C.
Optimal Reaction Temperature 42°C
Reverse Transcriptase SuperScript IV Reverse Transcriptase
Shipping Condition Dry Ice
Detection Method End point, real-time, lateral flow
Reaction Time Up to 20 min.
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For Research Use Only. Not for use in diagnostic procedures.