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  5. MilliporeSigma™ Immobilon Western HRP Chemiluminescence Substrates

MilliporeSigma™ Immobilon Western HRP Chemiluminescence Substrates

Catalog No. WBLUR0500
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Quantity:
100 mL
500 mL
Product Type:
HRP Substrate
Sensitivity:
∼1 to 3 pg
∼400 fg
∼6 pg
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Catalog No. Quantity Product Type Sensitivity
WBLUR0500 500 mL HRP Substrate ∼1 to 3 pg
WBLUF0500 500 mL HRP Substrate ∼400 fg
WBLUF0100A 100 mL HRP Substrate ∼400 fg
WBLUC0500 500 mL HRP Substrate ∼6 pg
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Catalog No. WBLUR0500 Supplier MilliporeSigma™ Supplier No. WBLUR0500
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Description

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.

  • Consistent results with less pipetting error

The stabilizers added to Luminata substrates:

  • Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
  • Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
  • Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation

Three different sensitivities for a range of detection needs:

  • Immobilon Classico premixed reagent for everyday detection needs
  • Immobilon Crescendo premixed reagent for detection in the picogram range
  • Immobilon Forte premixed reagent for detection in the femtogram range

Specifications

Product Type HRP Substrate
For Use With (Application) Dot/Slot Blot
Quantity 500 mL
Sensitivity ∼1 to 3 pg
Content And Storage 2°C to 8°C