Invitrogen™ Low Fluorescence PVDF Membranes

Description

Invitrogen Low Fluorescence PVDF membranes are specifically optimized to provide exceptional performance in western blot, fluorescent total protein normalization, and dot blot applications. These membranes maintain high protein binding characteristics while providing minimal auto-fluorescence across a wide range of excitation wavelengths (280–800 nm). As a result, Low Fluorescence PVDF membranes provide excellent multi-plexing capability and help achieve higher signal-to-noise ratios and improved detection limits across a broad spectrum of fluorescent dyes.

Features and benefits

• Low background autofluorescence—reduced background noise across multiple wavelengths, providing superior signal-to-noise
• Consistency and reproducibility—optimized pore size engineered to produce exceptional results, regardless of protein size and abundance
• Versatile formats—assortment of sizes and formats to match specific laboratory needs

Find the perfect format

Low Fluorescence PVDF membranes are available in rolls, pre-cut sheets, and pre-assembled membrane and filter paper sandwiches. The single-gel width roll can be easily cut to length for use with either mini or midi gel transfers. Precut sizes are available for both mini and midi gels, reducing handling effort and saving time. Compatible with wet tank transfer and semi-dry transfer systems, Low Fluorescence PVDF membranes pair with any preferred protein gel, helping improve data quality without changing the workflow.

Optimized pore size

Low Fluorescence PVDF membranes are manufactured with a narrower distribution of pore sizes compared to conventional PVDF membranes. The pore distribution of our 0.3 μm Low Fluorescence PVDF membranes is optimized to deliver consistent and reproducible results for western blotting applications, irrespective of target protein size. Low Fluorescence PVDF membranes facilitate uniform protein transfer and detection, helping ensure high-quality, reliable western blot data.

Applications

• Fluorescent western blotting with IR and RGB fluorophores
• Total protein normalization and quantitative western blotting
• Chemiluminescent western blotting