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Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green DFS Item

Determine the viability of cells quickly and easily. Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green is a ready-to-use assay kit for use in for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.

Manufacturer:  Invitrogen™ LSR37609

Catalog No. R37609



Includes: 3 x 2.5mL NucBlue Live, 3 x 2.5mL NucGreen Dead

Description

Description

ReadyProbes™ Cell Viability Imaging Kit, Blue/Green contains NucBlue™ Live reagent (Hoechst 33342) and NucGreen™ Dead reagent. Just add 2 drops of each stable reagent (at room temperature) to 1mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. NucGreen™ Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC/GFP (green) filter set.

  • NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460nm)
  • NucGreen™ Dead reagent: stains only the nuclei of dead cells with compromised plasma membranes; detected with standard FITC/GFP (green) filter set (excitation/emission maxima: 504/523nm)

Suggestions for use

  • NucBlue™ Live and NucGreen™ Dead reagents may be added directly to cells in full growth media or a compatible buffer solution.
  • In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
Specifications

Specifications

Store at 2° to 8°C, or at room temperature.
Determining the viability of cells
Blue/Green
Mammalian Cells, Eukaryotic Cells
Fluorescence Microscope
3 x 2.5 mL NucBlue™ Live, 3 x 2.5 mL NucGreen™ Dead
Dropper bottles
3 x 2.5 mL NucBlue® Live
3 x 2.5 mL NucGreen® Dead

Store at 2–8°C, or room temperature.
Fluorescence
SDS
Documents