Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green
Determine the viability of cells quickly and easily. Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green is a ready-to-use assay kit for use in for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.
Manufacturer: Invitrogen™ LSR37609
ReadyProbes™ Cell Viability Imaging Kit, Blue/Green contains NucBlue™ Live reagent (Hoechst 33342) and NucGreen™ Dead reagent. Just add 2 drops of each stable reagent (at room temperature) to 1mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. NucGreen™ Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC/GFP (green) filter set.
- NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460nm)
- NucGreen™ Dead reagent: stains only the nuclei of dead cells with compromised plasma membranes; detected with standard FITC/GFP (green) filter set (excitation/emission maxima: 504/523nm)
Suggestions for use
- NucBlue™ Live and NucGreen™ Dead reagents may be added directly to cells in full growth media or a compatible buffer solution.
- In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
|Store at 2° to 8°C, or at room temperature.|
|Determining the viability of cells|
|Mammalian Cells, Eukaryotic Cells|
|3 x 2.5 mL NucBlue™ Live, 3 x 2.5 mL NucGreen™ Dead|
|3 x 2.5 mL NucBlue® Live
3 x 2.5 mL NucGreen® Dead
Store at 2–8°C, or room temperature.