missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. Cell Function Reagents and Kits
  6. R37609

Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green

Numéro de catalogue. R37609
Encompass
Change view
Click to view available options
Quantity:
1 kit
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Numéro de catalogue. Quantity
R37609 1 kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Numéro de catalogue. R37609 Fournisseur Invitrogen™ Code fournisseur R37609
Il en reste null

Description

Comprend

3 x 2.5mL NucBlue Live, 3 x 2.5mL NucGreen Dead

Determine the viability of cells quickly and easily. Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green is a ready-to-use assay kit for use in for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.

ReadyProbes™ Cell Viability Imaging Kit, Blue/Green contains NucBlue™ Live reagent (Hoechst 33342) and NucGreen™ Dead reagent. Just add 2 drops of each stable reagent (at room temperature) to 1mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. NucGreen™ Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC/GFP (green) filter set.

  • NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460nm)
  • NucGreen™ Dead reagent: stains only the nuclei of dead cells with compromised plasma membranes; detected with standard FITC/GFP (green) filter set (excitation/emission maxima: 504/523nm)

Suggestions for use

  • NucBlue™ Live and NucGreen™ Dead reagents may be added directly to cells in full growth media or a compatible buffer solution.
  • In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.

Spécifications

Cell Type Mammalian Cells, Eukaryotic Cells
Color Blue, Green
Content And Storage Store at 2° to 8°C, or at room temperature.
Description ReadyProbes Cell Viability Imaging Kit, Blue/Green
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope
Product Type Cell Viability Imaging Kit
Dye Type 3 x 2.5 mL NucBlue™ Live
Emission 360/504
Excitation Wavelength Range 460, 523 nm
Format Dropper bottles
Product Line ReadyProbes
Quantity 1 kit
Shipping Condition Room Temperature
Afficher plus de résultats Afficher moins de résultats

For Research Use Only. Not for use in diagnostic procedures.