missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. PeproTech LIF Polyclonal Antibody, PeproTech®, Invitrogen™

PeproTech LIF Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P391MG
Encompass
Change view
Click to view available options
Quantity:
100 μg
50 μg
1 mg
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
500P391MG 1 mg
500P3950UG 50 μg
500P39100UG 100 μg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Catalog No. 500P391MG Supplier PeproTech Supplier No. 500P391MG
Only null left

Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human LIF. Anti-Human LIF-specific antibody was purified by affinity chromatography employing an immobilized Human LIF matrix. Sandwich ELISA: To detect Human LIF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human LIF (500-P39Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human LIF. Western Blot: To detect Human LIF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LIF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

Specifications

Antigen LIF
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene Lif
Gene Accession No. P15018
Gene Alias CDF; cholinergic differentiation factor; cholinergic neuronal differentiation factor; d factor; DIA; differentiation inhibitory activity; differentiation-inducing factor; differentiation-stimulating factor; Emfilermin; hepatocyte-stimulating factor III; HILDA; human interleukin in DA cells; leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); LIF; Melanoma-derived LPL inhibitor; MLPLI; myeloid leukaemia inhibitory factor
Gene Symbols Lif
Host Species Rabbit
Immunogen E.coli-derived Recombinant Human LIF.
Purification Method Antigen affinity chromatography
Quantity 1 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3976
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
Show More Show Less