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Thermo Scientific™ LanthaScreen™ TR-FRET VDR Coactivator Assay Kit, goat

Catalog No. PV4554
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800 x 20 μL assays
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PV4554 800 x 20 μL assays
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Catalog No. PV4554 Supplier Thermo Scientific™ Supplier No. PV4554
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Description

Contains Goat Tb-Anti-GST antibody

  • This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15127
  • The LanthaScreen™ TR-FRET Vitamin D Receptor (VDR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential VDR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a VDR ligand-binding domain (VDR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format
  • To run the LanthaScreen™ TR-FRET VDR Coactivator Assay, VDR-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.
  • Based upon the biology of the VDRcoactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
  • The LanthaScreen™ TR-FRET VDR Coactivator Assay Kit contains the VDR-LBD (GST) protein, fluorescein-labeled TRAP220/DRIP-2 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Specifications

Conjugate Tb (Terbium)
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Quantity 800 x 20 μL assays
Packaging 384-well Plate
Content And Storage Store in ultra-cold freezer (-68 to -85°C).
Assay Entry Biochemical coregulator interaction
Gene ID (Entrez) 7421
Readout End Point
Shipping Condition Dry Ice
Target Entry VDR, NR1I1
Product Line LanthaScreen
For Use With (Application) Co-Factor Interaction Assay, TR-FRET
Ligand VDR
Product Type TR-FRET VDR Coactivator Assay Kit
No. of Assays 800 x 20 μL Assays
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For Research Use Only. Not for use in diagnostic procedures.