missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PV4385

Thermo Scientific™ LanthaScreen™ TR-FRET RXR beta Coactivator Assay Kit, goat

Catalog No. PV4385
Encompass
Change view
Click to view available options
Quantity:
800 x 20 μL Assays
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PV4385 800 x 20 μL Assays
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PV4385 Supplier Thermo Scientific™ Supplier No. PV4385
Only null left

Description

Contains Goat Tb-Anti-GST antibody

  • This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15120:
  • The LanthaScreen™ TR-FRET Retinoid X Receptor (RXR) beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RXR beta ligands as agonists of coactivator recruitment. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
  • The LanthaScreen™ TR-FRET RXR beta Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human RXR beta ligand-binding domain (RXR beta-LBD) that is tagged with glutathione-S-transferase (GST). To assay, the RXR beta-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound
  • The LanthaScreen™ TR-FRET RXR beta Coactivator Assay Kit contains RXR beta-LBD (GST) protein, fluorescein-labeled D22 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Specifications

Conjugate Tb (Terbium)
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Quantity 800 x 20 μL Assays
Packaging 384-well Plate
Content And Storage Store in ultra-cold freezer (-68 to -85°C).
Assay Entry Biochemical coregulator interaction
Gene ID (Entrez) 6257
Readout End Point
Shipping Condition Dry Ice
Target Entry RXR beta, NR2B2, RXRB
Product Line LanthaScreen
For Use With (Application) Co-Factor Interaction Assay, TR-FRET
Ligand RXR beta
Product Type TR-FRET RXR Beta Coactivator Assay Kit
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.