missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Immunohistochemistry Kits and Reagents
  5. A15142

Thermo Scientific™ LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, rabbit

Catalog No. A15142
Encompass
Change view
Click to view available options
Quantity:
800 x 20 μL assays
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
A15142 800 x 20 μL assays
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. A15142 Supplier Thermo Scientific™ Supplier No. A15142
Only null left

Description

The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR).

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4839:

The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:

When running the LanthaScreen™ TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound's ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, 'addition-only' format.

Specifications

Color Green
Conjugate Tb (Terbium)
Description LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, Rabbit, 800 x 20μL Assays, Green, 384-Well Plate Format, Fluorescent Detection Method, Nuclear Receptors Druggable Target, Tb (Terbium) Label, TR-FRET Readout, Nuclear Receptor Assay
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Includes Tb-anti-GST Antibody (Rabbit), PXR(SXR)-LBD, GST, fluormone PXR(SXR) green, TR-FRET PXR(SXR) assay buffer, 1M DTT
Ligand Coupling Method Competitive ligand binding
Quantity 800 x 20 μL assays
Regulatory Status For research use only. Not for use in diagnostic procedures
Packaging 384-well Plate
Storage Buffer TR-FRET PXR(SXR) assay buffer
Content And Storage • Tb-anti-GST Antibody (Rabbit) (store at -20°C)
• PXR(SXR)-LBD, GST (-80°C)
• Fluormone PXR(SXR) Green (-20°C)
• TR-FRET PXR(SXR) Assay Buffer (4°C)
• 1M DTT (-20°C or -80°C)
Assay Category Biochemical Assay
Druggable Target Nuclear Receptors
Readout TR-FRET (Binding)
Product Line LanthaScreen
For Use With (Application) Nuclear Receptor Assay, TR-FRET
Ligand PXR
Product Type TR-FRET PXR (SXR) Competitive Binding Assay Kit
No. of Assays 800 x 20 μL
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.