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Thermo Scientific™ LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit, rabbit 
The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment.
Supplier: Thermo Scientific™ A15128
Description
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4655:
The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR alpha ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.
In the LanthaScreen™ TR-FRET LXR alpha Coactivator Assay, LXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
Specifications
| Green | |
| LanthaScreen™ TR-FRET LXR Alpha Coactivator Assay Kit, 800 x 20μL Assays, Green, 384-Well Plate Format, Tb (Terbium) Dye Type, Fluorescent Detection Method, TR-FRET Technique, For Pharma & Biopharma, Target & Lead Identification, Validation | |
| Microplate Reader | |
| 800 x 20 ÎĽL assays | |
| 384-well Plate | |
| Biochemical Assay | |
| TR-FRET (Activity) | |
| Nuclear Receptor Assay, TR-FRET | |
| TR-FRET LXR Alpha Coactivator Assay Kit |
| Tb (Terbium) | |
| Fluorescence | |
| Tb-anti-GST antibody (rabbit), LXR alpha-LBD, GST, fluorescein coactivator peptide, TR-FRET coregulator buffer H, 1M DTT | |
| For research use only (RUO) | |
| • Tb-anti-GST Antibody (Rabbit) (store at -20°C) • LXR alpha-LBD, GST (-80°C) • Fluoroscein Coactivator Peptide (-20°C) • TR-FRET Coregulatore Buffer H (4°C) • 1M DTT (-20°C or -80°C) |
|
| Nuclear Receptors | |
| LanthaScreen™ | |
| LXR alpha | |
| 800 x 20 ÎĽL Assays |
For Research Use Only. Not for use in diagnostic procedures.