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Gibco™ KnockOut™ Serum Replacement
Description
Gibco KnockOut Serum Replacement (KnockOut SR) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this serum replacement has been trusted by many for almost 20 years. Providing the documents required for regulatory review, KnockOut SR reduces your burden in qualifying reagents when transitioning from research applications to clinical applications.
With KnockOut Serum Replacement, you can:
- Culture feeder-dependent PSCs with confidence
- Rely on the improved performance of a qualified, FBS-free formulation
- Enjoy the peace of mind of having a more stable price and supply compared to FBS
Culture PSCs with confidence
KnockOut SR has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). It has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.
Rely on improved performance
KnockOut SR has more a defined formulation compared to FBS, which contains many undefined and variable components. Unlike most FBS products, each lot is tested for its ability to support PSCs. Because of this, culturing PSCs with KnockOut SR leads to less differentiation and more compact colony morphology compared to FBS.
Enjoy the peace of mind of having stable price and supply
KnockOut SR is not affected by the limited supply and fluctuating prices often seen with FBS. This makes it easier to plan your budget and buy what you need, especially since it is more affordable than most FBS products.
Specifications
Specifications
| Classification | Serum-Free |
| Content And Storage | Store in freezer (-5 to -20°C) and protect from light. |
| Culture Type | Mammalian Cell Culture |
| Species | Serum-Free |
| Cell Type | Stem Cells (Embryonic) |
| Form | Frozen |
| Serum Level | Serum-Free |
| Product Type | Serum Replacement |
| Quantity | 100 mL |
| Tested For | Growth Performance Assay |
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Frequently Asked Questions (FAQs)
Yes, mouse embryonic stem cells can be cultured using KnockOut SR under feeder-free conditions. Typically, cells are plated at a higher seeding density, in the presence of LIF, on a 0.1% gelatin layer in place of feeder cells.
Yes, KnockOut SR was designed to culture mouse ES cells in undifferentiated conditions.
Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).
Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).
Embryonic stem (ES) cells are derived from the early mammalian embryo and are capable of unlimited, undifferentiated proliferation in vitro while maintaining their potential to differentiate into a wide of range of adult tissues including germ cells. The pluripotency of the ES cells is normally demonstrated in vitro by inducing ES cells to differentiate into embryoid bodies and checking lineage-specific markers for differentiated cells in three body layers (endo, meso, and ectoderm), or injecting them into immunodeficient mice and determining the cell types produced in the teratomas.
For research use only. Not for use in diagnostic procedures.