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  5. Thermo Scientific™ KingFisher™ Flex Purification System

Thermo Scientific™ KingFisher™ Flex Purification System

Catalog No. A39966
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96 KF PCR Head
96 Plate and Warranty
96 Plate with SmartStart
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Catalog No. Product Type
A39966 96 Plate with SmartStart
501527923 96 KF PCR Head
A39952 96 Plate and Warranty
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Catalog No. A39966 Supplier Thermo Scientific™ Supplier No. A39966

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Prepare samples efficiently from a variety of materials with Thermo Scientific KingFisher Flex Purification Systems, which offer highly versatile, automated magnetic-particle processing for DNA/RNA, protein or cell purification from virtually any source.

Prepare samples efficiently from a variety of materials with Thermo Scientific KingFisher Flex Purification Systems, which offer highly versatile, automated magnetic-particle processing for DNA/RNA, protein or cell purification from virtually any source. Using revolutionary magnetic particle separation technology, this system provides excellent reproducibility and quality.

Find a flexible solution for different types of sample processing needs. With 24 configurations, customers can raise the processing volume up to 5 mL, resulting in increased yield of the purified product. For higher throughput needs, 96 samples can be processed with 96 magnet head.

  • Fully automated system yields high-speed purification of nucleic acids, proteins and cells.
  • High throughput system processes up to 96 samples in less than 20 minutes.
  • Expands sample volume by 10-fold with the 24-well format.
  • Open and flexible system lets the customer use any magnetic particle-based kit to meet the application demands.
  • Easy-to-use Thermo Scientific™ BindIt™ Software provides instrument control, protocol creation andmodification.
  • Ready-made protocols for different type of applications are available.

Recommended for:

  • Genomics and proteomics
  • Target identification
  • Veterinary assays
  • Biomarker discovery
  • Quality control
  • Forensics

Specifications

Product Type 96 Plate with SmartStart
Volume (Metric) Processing 20 to 200 μL
Plates per Deck 24 or 96
Format 96-well Plate
Capacity 96 Samples
Collection Efficiency of Particles > 95%
Display START / PAUSE / STOP / OK / TURNTABLE ROTATING CLOCKWISE / TURNTABLE ROTATING COUNTERCLOCKWISE / Four cursor keys / LCD
For Use With (Application) Genomics and proteomics, target identification, veterinary assays, biomarker discovery, quality control, forensics
Heating Temperature From +5°C above ambient temperature to +115°C
Magnetic Rods 96 in one frame; Interchangeable magnet heads
Particle Size (Metric) ca. >1 μm
Quantity System + SmartStart
Plate Type 24 or 96-well plates; KingFisher 24 Deep Well plate (200 to 5000 μL); Microtiter 96 Deep Well plate (50 to 1000 μL); KingFisher 96 plate (20 to 200 μL); PCR plate (20 to 100 μL)
Depth (English) 23.6 in.
Depth (Metric) 60 cm
Height (English) 15 in.
Height (Metric) 38 cm
Weight (English) 62 lb.
Weight (Metric) 28 kg
Width (English) 26.8 in.
Width (Metric) 68 cm
Customer Education 1 day at customer site
Total Service Coverage 1 year manufacturer warranty
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Frequently Asked Questions (FAQs)

Where can I find the manual for the BindIt Software?

The User Manual for the BindIt Software can be downloaded from the BindIt Software product page online, under the Documents & Downloads section. It is also included within the BindIt Software installation file under "Documentation".

I cannot connect my KingFisher Flex instrument to the BindIt software. How do I troubleshoot this issue?

To determine the cause of the connection issue, we recommend checking the following:
1. Check software settings:
• Make sure the instrument selected is KingFisher Flex.
• Make sure the instrument serial number was input with the dash included and no spaces (ex, "711-xxxx").
• Make sure the communication tab was set to "USB" and not "COM".
2. Check the instrument and computer:
• The KingFisher Flex instrument screen (GUI) should be set to the "Factory Protocols" tab (blue tab) for best results.
• Make sure the port function on the Maintenance Tab (last tab) is checked for "USB".
• Try using different USB cables.
• Try a different USB port on the computer.
• Check the Windows version you are using.
• Reboot the KingFisher Flex instrument and the computer.
• Make sure the driver was installed properly. This may require the help of your local IT team.

If the connection issue persists, please contact our Technical Support team at techsupport@thermofisher.com.

What Uninterruptible Power Supply (UPS) do you recommend I use with the KingFisher Flex instrument?

You can use any brand of UPS as long as it is compatible with the following KingFisher Flex instrument electrical specifications:
• Mains power supply: 100–240 Vac, 50/60 Hz, nominal automatic voltage detection
• Power consumption: 175 VA max.; 55 VA standby

I am getting the following error message on the KingFisher Flex instrument: error message 12. What can I do to fix this error?

The “error message 12” error usually means there is something out of place on the KingFisher Flex instrument. We recommend the following to resolve the issue:
1. Check the heat block and make sure that it is not lifted up out of the base of the unit. If it is out of the base, check to see if there is anything underneath it that is preventing it from going down. If there is nothing under the heat block, it may have gotten stuck in the up position. This will require a service call.
2. Check that the tip shield is off to the right and out of the way from underneath the magnetic head. If it is stuck in the incorrect position, this may require a service call.
3. If the turntable was moved and it clicked out of place, the rectangular footprint cannot be centered below the head. Turn off the instrument and turn it back on. The instrument should re-align itself.

If the error continues to display, please contact techsupport@thermofisher.com for assistance.

How do I use BindIt software 4.0 to load a script from my computer to the KingFisher Flex or KingFisher Duo/Duo Prime instrument?

Please follow these steps to load a script from your computer to the KingFisher Flex or KingFisher Duo/Duo Prime instrument:
1. Use the USB cable to connect the PC to the instrument.
2. Turn on the instrument.
3. Open the BindIt software and click the Connect button.
4. Select "Find New Instrument." BindIt will automatically connect to the instrument and the instrument screen will say "Computer Control".
5. Click the "Transfer" Button.
6. Click the "Upload" button.
7. Browse to find the file and double-click it.
8. In the new window that opens:
a. KingFisher Flex: you will be prompted to enter a new name – this is not necessary if the name only contains letters, digits, dash or underscore.
• If the name contains spaces, apostrophes or any other character, you should enter a new name containing only letters, digits, dash (-) or underscore (_).
• Click the "Category" pulldown to select the instrument folder you want to load the script to.
• Click OK.
b. KingFisher Duo/Duo Prime: The KingFisher Duo and Duo Prime instruments accept all characters in the filename.
• Click the "Category" pulldown to select the instrument folder you want to load the script to.
• Click OK.

I am using the KingFisher Flex instrument and see that there is a warning in the user guide that people wearing a pacemaker should not come into close contact with the magnet. What distance is safe for people with pacemakers to be at?

We recommend that people with pacemakers consult with their doctor before working with the KingFisher instruments. For the 24 Deep-Well Head, the magnetic force is 3,500 gauss. For the 96 Deep-Well Head, the magnetic force is 1,700 gauss. For the other magnetic heads, the magnetic force is lower.

Do the KingFisher Flex and KingFisher Duo instruments come with pre-loaded scripts?

The KingFisher Flex and KingFisher Duo instruments no longer come with pre-installed scripts. If your instrument begins with the following serial numbers, it will not have pre-loaded scripts:
• KingFisher Flex serial number 711-1951 or greater
• KingFisher Duo Prime serial number 706-1366 or greater

How can I calibrate the heating block for the KingFisher Flex instrument if it is off by 1 degree Celsius or more?

The accuracy of the KingFisher Flex heating block is ± 1°C for up to 80°C, and ± 2°C for up to 115°C (see page 72 of the manual). If there is an issue with the accuracy of the heating block, you can contact to schedule a service call. Calibration of the heating block can only be performed by one of our engineers.

Can I transfer a script protocol from my KingFisher Flex instrument back to the computer so that I can edit the protocol?

No, scripts cannot be transferred from the KingFisher Flex instrument to the computer for editing. It is possible to transfer a backup copy of the script from the KingFisher Flex instrument to the computer, but you will not be able to edit it. You may use this backup file to write a new script. In general, we recommend storing protocols in the BindIt software on the computer so the protocols can be edited there.

Do I receive training when I purchase the KingFisher Flex or KingFisher Duo Prime instruments?

No training is provided with the purchase of a KingFisher Flex or KingFisher Duo Prime instrument, but you can purchase training separately. We offer onsite half-day and full-day trainings for the KingFisher Flex and KingFisher Duo Prime instruments, depending on your needs. Please contact your local instrument sales representative to obtain a quote. The training will be led by your regional Field Application Scientist.

I am using a purification kit from a different company. Can you help me write a script to run on the KingFisher Flex instrument?

We cannot write a script for a purification kit for another company, but we can provide you with a tutorial on how to use the BindIt software. We also recommend checking with the manufacturer of the purification kit you are using, since some companies may already have scripts for their products to be used on multiple instruments.

Where can I find a script for processing a large volume of blood using the MagMAX Multi-Sample Ultra kit (Cat. No. A25597) with the KingFisher Flex or KingFisher Duo Prime instruments?

Scripts for processing large volumes of whole blood (up to 1 mL) can be found on the MagMAX Multi-Sample Ultra kit product page under the Product Literature section at the bottom of the page. There are two scripts available:
• KingFisher DUO file: A25597_Large_Volume_Blood.bdz
• KingFisher Flex file: A25597_Large_Volume_Blood.bdz

*Use the page scrolling function to see the complete list of product literature for this product.

What kind of training do you offer for the KingFisher Flex instrument and how much does it cost?

We offer onsite half-day and full-day trainings for the KingFisher Flex instruments, depending on your needs.The training will be led by your regional Field Application Scientist. You can contact your local sales representative to obtain a quote for the training cost.

What should I do if the tip comb does not load correctly onto my Thermo Scientific KingFisher system?

If the tip does not load correctly onto a Thermo Scientific KingFisher magnetic head, we recommend that you try manually flexing the tips both lengthwise and widthwise to level the tip comb. A video of flexing the tip comb can be found here: https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/kingfisher-systems/resources.html. The video is under "KingFisher Flex" and titled "How to install the magnet head and check the tip comb." Flexing of the tip comb is demonstrated at timepoint 1:35. If that does not work, try a different box or lot of tips. This applies to all KingFisher instruments.
Can I use the KingFisher instruments for plasmid purification?

We recommend using the MagJet Plasmid DNA kit (Cat. No. K2791) for plasmid purification. Scripts for using the MagJet Plasmid DNA kit with the KingFisher Flex and KingFisher Duo Prime Purification Systems are available for download on the kit product page (https://www.thermofisher.com/order/catalog/product/K2791). The ChargeSwitch Plasmid ER Mini Kit (Cat. No. CS10100) is also an option for plasmid purification. There is no script currently available for using ChargeSwitch Plasmid ER Mini Kit, but you can create a custom protocol for use with the KingFisher Flex and KingFisher Duo Prime Purification Systems.
Are the magnets on the KingFisher Flex Purification System magnetic head strong enough to disturb nearby laboratory equipment?

We do not recommend placing the KingFisher Flex Purification System in close proximity to magnetic tapes, computer discs or other magnetic storage systems, such as credit cards, as these can be damaged by the strong magnetic field of the KingFisher Flex magnetic heads. Do not hold the KingFisher Flex magnetic heads close to a PC display, since this may cause damage to the display. Do not use metal tools when handling KingFisher Flex magnetic heads. People wearing a pacemaker or metallic prostheses should also not use this product as these items could be affected or damaged by close contact with a strong magnetic field.

How should I clean the cover on the KingFisher Flex instrument?

We recommend cleaning the KingFisher Flex instrument cover with water containing a mild soap or a slightly acidic, neutral or slightly alkaline detergent. You can use up to a 3.5% Sodium Hypochlorite concentration. We do not recommend using phenol or chloroform to clean the cover.
What material is the KingFisher Flex Purification System cover made of?

The KingFisher Flex Purification System cover is made of polyethylene terephthalate (PET).
How does the KingFisher Flex Purification heating block work?

The KingFisher Flex Purification System heating block is located inside the instrument and can be used automatically during the protocol. All KingFisher Flex plates can be heated using specially designed, interchangeable heating blocks. Any number of heating steps can be added to a protocol. When a protocol enters a heating step, the plate is automatically moved to the dedicated heating position for heating. Afterwards, the protocol continues automatically to the next step.

Which software should I use for transferring scripts to my MagMAX Express-96 or KingFisher Flex instrument?

We recommend using the BindIt Software to transfer scripts to the MagMAX Express-96 and KingFisher Flex instruments. The BindIt Software should be used to transfer scripts for all of the Applied Biosystems MagMAX Express and Thermo Scientific KingFisher instruments.

For Laboratory Use