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Invitrogen™ T-REx™ Core Kit, with pcDNA™4/TO/myc-His© Vector 
Tetracycline-regulated expression system without viral transactivators
Supplier: Invitrogen™ K103002
Includes: 20μg of pcDNA4/TO or 20μg each of pcDNA4/TO/myc-His A, B, and C; a positive control vector, pcDNA6/TR, and forward and reverse sequencing primers. All vectors are supercoiled and provided lyophilized.
Description
- Uses complete CMV promoter and adds control elements from bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of strongest mammalian promoter sequences known
- Specific Activation: uses repressor mechanism that blocks transcription from powerful CMV promoter in absence of tetracycline
- Because T-REx System elements do not use viral transactivators, can achieve high-level expression from complete CMV promoter without secondary, non-specific activation of host genes
- Two tetracycline operator sequences (TetO2) have been inserted between TATA box of CMV promoter and transcriptional start site
- TetO2 sequence itself has no effect on expression: When tetracycline repressor protein (TR) is present, it effectively binds TetO2 sites and blocks transcription initiation
- Tetracycline added to culture medium binds to, and changes conformation of, TR protein causing TR protein to release TetO2 sites and derepressing transcription from CMV promoter: The result is high-level expression of gene of interest
- Expression levels can be modulated based on tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors
- Complete CMV enhancer-promoter sequence containing two copies of tetracycline operator TetO2 sequence for high-level regulated expression
- Zeocinor hygromycin resistance gene for effective selection of stable mammalian cell lines
- Large multiple cloning site to simplify cloning
- In addition, pcDNA™4/TO/myc-His offers a c-myc epitope for rapid detection of recombinant protein with Anti-myc Antibody and polyhistidine (6xHis) sequence for simple purification of recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody
- Regulatory vector, pcDNA™6/TR, is provided for high-level expression of tetracycline repressor (TR) protein
- Vector expresses Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express TR protein
Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression
Greener Choice Claims
- Environmental benefits include:
- Recyclable
Specifications
| Inducible | |
| Tetracycline | |
| T-Rex Core Cloning Kit with Vector | |
| The T-REx™ Core Kit includes 20 μg each of pcDNA™4/TO/myc-His A, B, and C; a positive control vector; pcDNA™6/TR; and forward and reverse sequencing primers. All vectors are supercoiled and provided lyophilized. Store all components at -20°C. All components are guaranteed stable for 6 months when properly stored. |
|
| Restriction Enzyme/MCS | |
| pcDNA | |
| Regulated Expression |
| Transfection | |
| CMV/TO | |
| Zeocin™ | |
| His Tag (6x), c-Myc Epitope Tag | |
| 1 kit | |
| T-REx™, pcDNA™ |
For Research Use Only. Not for use in diagnostic procedures.