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Invitrogen™ SuperScript™ III Reverse Transcriptase
Proprietary mutant of SuperScript II RT
$165.00 - $2,140.00
Specifications
Concentration | 200 U/μL |
---|---|
Final Product Type | First-Strand cDNA |
Format | Reverse Transcriptase |
GC-Rich PCR Performance | High |
PCR Method | RT-PCR, RT-qPCR |
Description
SuperScript III Reverse Transcriptase (RT) is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. Like SuperScript II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. SuperScript III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.- Thermostability: half-life of 220 minutes at 50°C
- Yield: reduced RNase H activity for more full-length cDNA
- Specificity: full activity at 50°C for increased specificity with gene-specific primers
- Applications—array labeling, cDNA libraries, RT-PCR, primer extension, 3' and 5' RACE
- Source: Purified from E. coli expressing the pol gene of M-MLV, mutagenized to increase thermal stability and half-life and to reduce RNase H activity
- Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
- Concentration: 200U/μL
- Unit definition: One unit of SuperScript III RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)·oligo(dT)12-18 as a template·primer
- Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)12-18, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C
- Optimal reaction temperature: 50°C
- Final product size: 12.3kb or less
PCR and Real-Time PCR, Reverse Transcription, array labeling, cDNA libraries, primer extension, 3' and '5 RACE
Order Info
Shipping Condition: Dry ice
Specifications
200 U/μL | |
Reverse Transcriptase | |
RT-PCR, RT-qPCR | |
30 to 50 min. | |
Up to 12.3 kb | |
Reverse Transcription | |
SuperScript III | |
Dry Ice |
First-Strand cDNA | |
High | |
Separate components | |
Reverse Transcription | |
RNA | |
50°C | |
Reduced |
For Research Use Only. Not for use in diagnostic procedures.