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Invitrogen™ SuperScript™ III Reverse Transcriptase DFS Item

Proprietary mutant of SuperScript II RT

$155.40 - $1,346.10

Specifications

Includes SuperScript III Reverse Transcriptase (4 × 10,000 units total, at 200U/μL), (4 × 1mL) of 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and (4 × 500μL) of 100mM DTT
Packaging Type Tube
Storage Requirements Store at -20deg.C
Temperature (English) Operating 122deg.F
Temperature (Metric) Operating 50deg.C
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

18080044

DFS Item
 
life technologies
18080044
50 reactions Each for $372.75

18080085

DFS Item
 
life technologies
18080085
200 reactions Each for $1,346.10

18080093

DFS Item
 
life technologies
18080093
10 reactions Each for $155.40
Description & Specifications

Specifications

Includes SuperScript III Reverse Transcriptase (4 × 10,000 units total, at 200U/μL), (4 × 1mL) of 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and (4 × 500μL) of 100mM DTT
Packaging Type Tube
Storage Requirements Store at -20deg.C
Temperature (English) Operating 122deg.F
Temperature (Metric) Operating 50deg.C
Test Sensitivity Medium

SuperScript III Reverse Transcriptase (RT) is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. Like SuperScript II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. SuperScript III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.

  • Thermostability: half-life of 220 minutes at 50°C
  • Yield: reduced RNase H activity for more full-length cDNA
  • Specificity: full activity at 50°C for increased specificity with gene-specific primers
    Applications—array labeling, cDNA libraries, RT-PCR, primer extension, 3' and 5' RACE
  • Source: Purified from E. coli expressing the pol gene of M-MLV, mutagenized to increase thermal stability and half-life and to reduce RNase H activity
  • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
  • Concentration: 200U/μL

  • Unit definition: One unit of SuperScript III RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)·oligo(dT)12-18 as a template·primer
  • Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)12-18, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C
  • Optimal reaction temperature: 50°C
  • Final product size: 12.3kb or less

PCR and Real-Time PCR, Reverse Transcription, array labeling, cDNA libraries, primer extension, 3' and '5 RACE

Shipping Condition: Dry ice