Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor 

Description

RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A and is used to avoid RNA degradation in a variety of applications. It is an acidic protein with a molecular weight of ∼52 kDa that inhibits RNase A, RNase B, and RNase C.

The robust molecular structure of the RNaseOUT™ inhibitor together with a very high binding affinity for pancreatic-type RNases ensures superior protection of RNA from degradation making RNaseOUT™ highly effective and reliable in various molecular biology applications.

Features

• Stable under prolonged reaction conditions—effective RNA protection from RNases at:
  • 4°C for at least 96 hours
  • 25°C for at least 96 hours
  • 37°C for 24 hours
  • 50°C for up to 6 hours
• Oxidation resistant—highly stable and requires low concentrations of DTT to retain RNase inhibiting activity
• Wide activity temperature range—maintains full activity from 4°C to 55°C
• Superior quality—stringent quality control and industry leading manufacturing process

Applications

• In vitro transcription and translation
• CRISPR guide RNA production
• RNA purification
• cDNA synthesis
• RT-PCR
• RT-LAMP
• RNA-seq
• RNA modification and labeling

Source

Purified by affinity chromatography from E. coli expressing a cloned porcine gene.

Performance and quality testing

• SDS-PAGE purity
• Endo-deoxyribonuclease assay
• Protein concentration
• Specific activity
• Performance evaluated by RT-PCR

Unit definition

One unit inhibits 5 ng of RNase A by 50% using cytidine 2', 3' cyclic monophosphate (cCMP) as the substrate.

Unit reaction conditions

100 mM Tris-acetate (pH 6.5), 1 mM EDTA, 0.2 mM cCMP, 2 μg RNase A in 1 mL from 0 to 10 min at 25°C