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Invitrogen™ PureLink™ Quick Gel Extraction and PCR Purification Combo Kit

Catalog No. K220001
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K220001 50 Preps
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Catalog No. K220001 Supplier Invitrogen™ Supplier No. K220001
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Description

Includes

The PureLink Quick Gel Extraction & PCR Purification Combo Kit includes sufficient PureLink columns, collection tubes, elution tubes, and reagents for 50 preps.

Offers ability to perform both a gel extraction or PCR purification with single kit.

PureLink™ Quick Gel Extraction and PCR Purification Combo Kit offers the ability to perform both a gel extraction or a PCR purification a single kit.

The PureLink™ Quick Gel Extraction and PCR Purification Combo Kit is designed to purify DNA fragments from agarose gels. The simple procedure uses a silica-based spin cartridge to purify DNA fragments from 40 bp to 10 kb in < 30 minutes. It also provide all components to perform a rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR fragments >100 bp in less than 10 minutes. Isolated DNA using the PureLink™ kit is free of proteins, dye, and agarose, and is ready-to-use for a variety of applications, including automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling. The kit contains all the buffers from the PureLink™ Quick Gel extraction kit and the PCR binding buffer from PureLink™ PCR Purification kit with Invitrogen branded lidded column.

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Isolation Technology Silica Spin Column
Scale Mini
Sample Type Linear DNA fragments from: Agarose Gels, PCR Purification, Restriction Enzyme Reactions, PCR Products
Elution Volume 50 μL
Final Product Type DNA
For Use With (Application) PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility Not High-throughput Compatible (Manual)
Label or Dye Ethidium Bromide, SYBR Safe
Quantity 50 Preps
Shipping Condition Room Temperature
Test Time Up to 30 min.
Yield 15 μg (Binding capacity)
Starting Material Amount Agarose Gel: ≤400 mg, PCR Product: ≤100 μL, or ≤40 μg dsDNA
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Frequently Asked Questions (FAQs)

I'm running an agarose gel with TAE or TBE. Can I use your gel extraction kits?

Yes, both TAE and TBE agarose gels are compatible with our gel extraction kits.
Can the PureLink Quick PCR Clean-Up Kit be used to purify linear/digested or supercoiled plasmid DNA?

Yes, you can purify ANY dsDNA sample with a size range of 100 bp to 12 kb from liquid samples with the PureLink Quick PCR Clean-Up Kit. Supercoiled DNA can be purified as well, but some nicking should be expected.
What is the difference between Purelink PureLink Quick Gel Extraction System and S.N.A.P. Gel Purification Kit?

The PureLink kit can be used with both TAE and TBE gels, while the S.N.A.P. Gel Purification Kit is recommended for TAE gels only.
What is unique about the S.N.A.P. UV-free Gel Purification Kit?

The S.N.A.P. UV-free Gel Purification Kit utilizes crystal violet as a staining reagent, which can be visualized in non-UV light conditions. This can prevent DNA damage that can be caused by the UV light required by normal ethidium bromide staining. DNA fragments recovered using this kit have higher cloning efficiency. In addition, DNA can be visualized under normal light as a thin violet band while the gel is running and excised as soon as bands are sufficiently resolved.

For Research Use Only. Not for use in diagnostic procedures.