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Invitrogen™ pPICZα A, B, & C Pichia Vectors 
Allows high-level, methanol inducible expression of the gene of interest in Pichia, and can be used in Pichia strains including X-33, SMD1168H, and KM71H
Supplier: Invitrogen™ V19520
Includes: 20μg each of lyophilized pPICZ A, B, and C is supplied
Description
- Recombinant proteins are expressed as fusions to an N-terminal peptide encoding Saccharomyces cerevisiae '-factor secretion signal
- pPICα vectors contain AOX1 promoter
- All three reading frames (A, B, C versions) are provided to facilitate in-frame cloning with C-terminal peptide
- α-factor secretion signal for directing secreted expression of recombinant protein
- Zeocin resistance gene for selection in both E. coli and Pichia
- C-terminal peptide containing c-myc epitope and polyhistidine (6xHis) tag for detection and purification of recombinant fusion protein
Pichia pastoris Expression Systems, Protein Expression, Proteins, Expression, Isolation and Analysis, Yeast Expression
Specifications
| AOX1 | |
| 20 μg each of lyophilized pPICZ A, B, and C is supplied (Store at -20C) | |
| Room Temperature | |
| Restriction Enzyme/MCS | |
| pPIC |
| Pichia Expression Vector | |
| Zeocin™ (ZeoR) | |
| His Tag (6x), c-Myc Epitope Tag | |
| 20 μg |
For Research Use Only. Not for use in diagnostic procedures.