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Invitrogen™ pMIB/V5-His Vector Kit
3.6kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five insect cells
Supplier: Invitrogen™ V803001
Comprend: 20μg each of supercoiled, lyophilized pMIB/V5-His A, B and C vectors, pMIB/V5-His/CAT control vector, and 2μg each of the OpIE2 Forward and Reverse Sequencing primers.
Description
- Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells
- The OpIE2 promoter for constitutive expression
- The honeybee melittin (HBM) secretion signal to allow secreted expression of the protein
- The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
- C-terminal V5-epitope and polyhistidine tag for detection with Anti-V5 antibody and purification with nickel-chelating resin
Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Secreted Protein Expression in Insect Cells
Spécifications
| OplE2 | |
| The pMIB/V5-His Vector Kit contains 20 μg each of supercoiled, lyophilized pMIB/V5-His A, B, and C vectors, pMIB/V5-His/CAT control vector, and 2 μg each of the OpIE2 Forward and Reverse Sequencing primers. Store at -20°C. All reagents are guaranteed stable for 6 months when properly stored. | |
| His Tag (6x), V5 Epitope Tag | |
| 1 kit | |
| InsectSelect |
| Insect Cell Expression Vector | |
| C-terminal | |
| Restriction Enzyme/MCS | |
| pMIB |
For Research Use Only. Not for use in diagnostic procedures.