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Invitrogen™ pGlow TOPO™ TA Expression Kit
Uses Cycle 3 GFP mutant as reporter protein for transcriptional activity
Supplier: Invitrogen™ K483001
Comprend: pGlow TOPO TA Expression Kits contains two boxes. The TOPO TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers. The box contains TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid.
Description
- Cycle 3 GFP is exellent for noninvasive analysis of promoter elements within intact, living cells Fluorescent property of Cycle 3 GFP allows
- in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods
Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g. GFP) Assays, Fluorescent Protein Assays (GFP)/Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays
Spécifications
| Transfection | |
| TOPO TA Expression Kit | |
| Geneticin™ (G-418) | |
| Untagged | |
| 20 reactions | |
| TOPO |
| None (Promoterless) | |
| GFP (Cycle 3) | |
| pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored. | |
| TOPO-TA | |
| pGlow, TOPO-TA Vectors | |
| Reporter Assays, Clone your own Promoter |
For Research Use Only. Not for use in diagnostic procedures.