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Invitrogen™ pcDNA™3.1/Zeo (+) Mammalian Expression Vector DFS Item

Designed for high-level, constitutive expression in a variety of mammalian cell lines

Supplier:  Invitrogen™ V86020

Catalog No. V86020


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Includes: 20ÎĽg of this pcDNA3.1/Zeo(+) vector, as well as an expression control, are supplied supercoiled and lyophilized.

Description

Description

  • Contains Zeocin selectable marker and forward-orientation multiple cloning site
  • Cytomegalovirus (CMV) enhancer-promoter for high-level expression
  • Large multiple cloning site in either forward (+) or reverse (-) orientations
  • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
  • Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

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Specifications

Specifications

Constitutive
CMV
Zeocin™
Untagged
20 ÎĽg
pcDNA™
Transfection
Mammalian Expression Vector
20 μg of this pcDNA™3.1/Zeo(+) vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.
Restriction Enzyme/MCS
pcDNA
Constitutive Expression
Product Suggestions

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Videos
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Documents

Documents

Product Information

Manuals

pcDNA3.1/Zeo(+) and pcDNA3.1/Zeo(-) Version: Version J 12 November 2010 File size: 386 kB
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For Research Use Only. Not for use in diagnostic procedures.