Invitrogen™ pcDNA™3.1/Zeo^(-) Mammalian Expression Vector

Designed for high-level, constitutive expression in a variety of mammalian cell lines

Supplier: Invitrogen™ V86520

Numéro de catalogue. V86520

Quantité

Comprend: 20μg of this pcDNA3.1/Zeo^(-) vector, as well as an expression control, are supplied supercoiled and lyophilized.

Description

Contains Zeocin selectable marker and reverse-orientation multiple cloning site
Cytomegalovirus (CMV) enhancer-promoter for high-level expression
Large multiple cloning site in either forward (+) or reverse (-) orientations
Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,COS-1 and COS-7)
Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Spécifications

Constitutive or Inducible System	Constitutive
Promoter	CMV
Selection Agent (Eukaryotic)	Zeocin™
Protein Tag	Untagged
Quantity	20 μg
Product Line	pcDNA

Delivery Type	Transfection
Product Type	Mammalian Expression Vector
Content And Storage	20 μg of this pcDNA™3.1/Zeo^(-) vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.
Cloning Method	Restriction Enzyme/MCS
Vector	pcDNA
For Use With (Application)	Constitutive Expression

missing translation for 'recommendations'

For Research Use Only. Not for use in diagnostic procedures.

Description