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Invitrogen™ pBAD/TOPO™ ThioFusion™ Expression Kit

Numéro de catalogue. K37001
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20 reactions
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Numéro de catalogue. Quantity
K37001 20 reactions
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Numéro de catalogue. K37001 Fournisseur Invitrogen™ Code fournisseur K37001
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Description

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All of the reagents necessary for PCR (except Taq polymerase) including 200ng of topoisomerase I-activated pBAD/Thio-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. The second box contains all of the reagents necessary for transformation including 21 single-use 50μL aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid.

Designed for one-step cloning and regulated expression of thioredoxin fusion proteins in E. coli

  • pBAD/Thio-TOPO vector encodes His-Patch thioredoxin as N-terminal fusion partner
  • Thioredoxin fusion can significantly increase solubility of many difficult-to-express proteins and improve yield of protein production
  • Vector features araBAD promoter for tightly regulated expression in E. coli I-activated vector for 5-minute TOPO Cloning of Taq-amplified PCR products
  • C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with aAnti-His(C-term) Antibody
  • C-terminal V5 epitope for detection with an Anti-V5 Antibody
  • Enterokinase cleavage site for efficient cleavage of N-terminal fusion tag with EKMaxEnterokinase

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Spécifications

Constitutive or Inducible System Inducible
Inducing Agent Arabinose
Promoter araBAD
Product Type Lentiviral Expression Kit
Selection Agent (Eukaryotic) None
Content And Storage Each pBAD/TOPO™ ThioFusion™ Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD/TOPO™ ThioFusion™ Expression box contains all of the reagents necessary for PCR (except Taq polymerase) including 200 ng of topoisomerase I-activated pBAD/Thio-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation including 21 single-use 50 μl aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store the One Shot™ box at -80°C. Store the LMG194 E. coli stab at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Bacterial or Yeast Strain TOP10
Cleavage EK (Enterokinase) Recognition Site
Expression System E. coli
Expression Mechanism Cell-Based Expression
Protein Tag His Tag (6x), V5 Epitope Tag, Thioredoxin
Cloning Method TOPO-TA
Quantity 20 reactions
Vector pBAD
Product Line TOPO, ThioFusion
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For Research Use Only. Not for use in diagnostic procedures.