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Invitrogen™ pBAD/TOPO™ ThioFusion™ Expression Kit
Designed for one-step cloning and regulated expression of thioredoxin fusion proteins in E. coli
Supplier: Invitrogen™ K37001
Includes: All of the reagents necessary for PCR (except Taq polymerase) including 200ng of topoisomerase I-activated pBAD/Thio-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. The second box contains all of the reagents necessary for transformation including 21 single-use 50μL aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid.
Description
- pBAD/Thio-TOPO vector encodes His-Patch thioredoxin as N-terminal fusion partner
- Thioredoxin fusion can significantly increase solubility of many difficult-to-express proteins and improve yield of protein production
- Vector features araBAD promoter for tightly regulated expression in E. coli I-activated vector for 5-minute TOPO Cloning of Taq-amplified PCR products
- C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with aAnti-His(C-term) Antibody
- C-terminal V5 epitope for detection with an Anti-V5 Antibody
- Enterokinase cleavage site for efficient cleavage of N-terminal fusion tag with EKMaxEnterokinase
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| araBAD | |
| None | |
| Ampicillin (AmpR) | |
| EK (Enterokinase) Recognition Site | |
| Cell-Based Expression | |
| TOPO-TA | |
| pBAD |
| Arabinose | |
| Lentiviral Expression Kit | |
| Each pBAD/TOPO™ ThioFusion™ Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD/TOPO™ ThioFusion™ Expression box contains all of the reagents necessary for PCR (except Taq polymerase) including 200 ng of topoisomerase I-activated pBAD/Thio-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation including 21 single-use 50 μl aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store the One Shot™ box at -80°C. Store the LMG194 E. coli stab at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored. | |
| TOP10 | |
| E. coli | |
| His Tag (6x), V5 Epitope Tag, Thioredoxin | |
| 20 reactions | |
| TOPO, ThioFusion |
For Research Use Only. Not for use in diagnostic procedures.