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Invitrogen™ pBAD/His Kit

Express the protein of interest with an N-terminal tag in a tightly regulated fashion

Supplier: Invitrogen™ V43001

Catalog No. V43001

Quantity

Includes: 20μg of each pBAD/His A, B & C vector, 20μg of pBAD/His/lacZ, 1mL of 20% L-arabinose, LMG194 and TOP10 E. coli stabs.

Description

araBAD promoter for tightly regulated expression
Translation initiation signals optimized for E. coli expression
N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with Anti-HisG Antibody
N-terminal Xpress epitope for detection and analysis with Anti-Xpress Antibody
Enterokinase cleavage site for removing the N-terminal tag following purification
Three vectors are provided (A, B, and C): Each has the N-terminal tag in a different reading frame relative to multiple cloning site to simplify in-frame cloning of gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Constitutive or Inducible System	Inducible
Promoter	araBAD
Selection Agent (Eukaryotic)	None
Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Protein Tag	His Tag (6x)
Quantity	1 kit

Inducing Agent	Arabinose
Product Type	Bacterial Expression Vector
Content And Storage	The pBAD/His Kit includes 20 μg of each pBAD/His A, B, & C vector, 20 μg of pBAD/His/lacZ, 1 ml of 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.
Cleavage	EK (Enterokinase) Recognition Site
Cloning Method	Restriction Enzyme/MCS
Vector	pBAD

Product Suggestions

For Research Use Only. Not for use in diagnostic procedures.

Description