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Invitrogen™ pBAD/gIII Kit

Express recombinant proteins under the tight regulation of the araBAD promoter

Supplier: Invitrogen™ V45001

Catalog No. V45001

Quantity

Includes: T20μg each of pBAD/gIII A, B and C, 20μg of pBAD/gIII/calmodulin, 1mL 20% L-arabinose, LMG194 and TOP10 E. coli stabs.

Description

To simplify purification, vector pBAD/gIII encodes a leader peptide which directs recombinant protein into the periplasmic space
Features araBAD promoter for tightly regulated expression
Translation initiation signals for optimized E. coliexpression
Leader peptide from bacteriophage fd gene III protein (gIII) for secreted expression
C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
A set of three vectors is provided (A, B, and C): Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of the gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Constitutive or Inducible System	Inducible
Promoter	araBAD
Selection Agent (Eukaryotic)	None
Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Cloning Method	Restriction Enzyme/MCS
Vector	pBAD

Inducing Agent	Arabinose
Product Type	Bacterial Expression Vector
Content And Storage	The pBAD/gIII Kit includes 20 μg each of pBAD/gIII A, B, & C, 20 μg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.
Protein Tag	c-Myc Epitope Tag
Quantity	1 kit

Product Suggestions

For Research Use Only. Not for use in diagnostic procedures.

Description