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Invitrogen™ pBAD/gIII Kit

Catalog No. V45001
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V45001 1 kit
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Catalog No. V45001 Supplier Invitrogen™ Supplier No. V45001
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Description

Includes

T20μg each of pBAD/gIII A, B and C, 20μg of pBAD/gIII/calmodulin, 1mL 20% L-arabinose, LMG194 and TOP10 E. coli stabs.

Express recombinant proteins under the tight regulation of the araBAD promoter

  • To simplify purification, vector pBAD/gIII encodes a leader peptide which directs recombinant protein into the periplasmic space
  • Features araBAD promoter for tightly regulated expression
  • Translation initiation signals for optimized E. coliexpression
  • Leader peptide from bacteriophage fd gene III protein (gIII) for secreted expression
  • C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
  • C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
  • A set of three vectors is provided (A, B, and C): Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of the gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Constitutive or Inducible System Inducible
Inducing Agent Arabinose
Promoter araBAD
Product Type Bacterial Expression Vector
Selection Agent (Eukaryotic) None
Content And Storage The pBAD/gIII Kit includes 20 μg each of pBAD/gIII A, B, & C, 20 μg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Protein Tag c-Myc Epitope Tag
Cloning Method Restriction Enzyme/MCS
Quantity 1 kit
Vector pBAD
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For Research Use Only. Not for use in diagnostic procedures.