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Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli DFS Item

Ideal for high-efficiency cloning and plasmid propagation

$588.00 - $1,043.70

Specifications

Bacterial Content TOP10F´
Cell Type Chemically Competent
Species E. coli
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

C303006

DFS Item
 
invitrogen™
C303006
40 x 50μL Each for $1,043.70

C303003

DFS Item
 
invitrogen™
C303003
20 x 50μL Each for $588.00
Description & Specifications

Specifications

Bacterial Content TOP10F´
Cell Type Chemically Competent
Species E. coli

One Shot TOP10F Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome. TOP10 competent cells are provided at a transformation efficiency of 1 x 109 cfu/μg supercoiled DNA.<br><br>Using One Shot TOP10F' Chemically Competent E. coli<br>The F' episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F' episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F cells require IPTG induction for blue/white color screening. High efficiency (> 109 cfu/μg).

Not high-throughput compatible (Manual)

Cloning, Transformation

Shipping Condition: Dry Ice