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Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli DFS Item

Ideal for high-efficiency cloning and plasmid propagation

$614.00 - $1,060.00


Bacterial Content TOP10F´
Cell Type Chemically Competent
Species E. coli
View More Specs


For Research Use Only. Not for use in diagnostic procedures.

Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
C303006 Invitrogen™
40 x 50μL Each for $1,060.00
C303003 Invitrogen™
20 x 50μL Each for $614.00


One Shot TOP10F Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome. TOP10 competent cells are provided at a transformation efficiency of 1 x 109 cfu/μg supercoiled DNA.

Using One Shot TOP10F' Chemically Competent E. coli
The F' episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F' episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F cells require IPTG induction for blue/white color screening. High efficiency (> 109 cfu/μg).

Not high-throughput compatible (Manual)

Cloning, Transformation

Order Info

Shipping Condition: Dry Ice

Description & Specifications


Chemically Competent
E. coli