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  5. 12537100

Invitrogen™ MultiSite Gateway™ Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway™ destination vector

Numéro de catalogue. 12537100
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Numéro de catalogue. Quantity
12537100 20 reactions
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Numéro de catalogue. 12537100 Fournisseur Invitrogen™ Code fournisseur 12537100
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Description

Comprend

Include vectors and primers, Mach1 T1R Chemically Competent Cells, and LR Clonase II Plus and BP Clonase II enzymes.

For flexible cloning of up to four DNA fragments into Gateway destination vector

  • Using specifically designed att sites for recombinational cloning, clone two, three, or four DNA fragments into any Gateway Destination vector containing attR1 and attR2 sites
  • Resulting expression clone is ready for downstream expression and analysis applications
  • Clone multiple DNA fragments into one vector without using restriction enzymes or ligases
  • Full advantage of wide selection of Gateway Destination vectors or create vectors
  • Replace multiple plasmid transfections with single vector carrying all DNA elements of interest
  • MultiSite Gateway Pro Kits are available for cloning two, three, or four DNA fragments and for flexible and customized configuration of DNA fragments into a Gateway Destination vector

Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Multi-Gene Expression, Multisite Gateway, Protein Expression, Proteins, Expression, Isolation and Analysis

Spécifications

Promoter None (Promoterless)
Product Type Gateway System Destination Expression Vector
Content And Storage The MultiSite Gateway™ Pro Kits include vectors and primers as indicated in Table 1, One Shot™ Mach1™ T1R Chemically Competent Cells, and LR Clonase™ II Plus and BP Clonase™ II enzymes. Store cells at -80°C. Store Clonase™ enzymes at -20°C for up to 6 months, or at -80°C for long-term storage. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Cloning Method Gateway
Quantity 20 reactions
Vector MultiSite Gateway Vectors
Product Line Gateway
For Use With (Application) Clone your own Promoter, Multi-Gene Expression

For Research Use Only. Not for use in diagnostic procedures.