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Invitrogen™ M-MLV Reverse Transcriptase (200 U/µL) 
Recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid
$486.00 - $2,205.00
Specifications
| Concentration | 200 U/μL |
|---|---|
| Final Product Type | First-Strand cDNA |
| Format | M-mLV Reverse Transcriptase |
| PCR Method | RT-PCR, RT-qPCR |
| Reaction Format | Separate components |
Description
Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.- Thermostability—optimal activity at 37°C
- Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7kb
- Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
- Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid
- Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
- Concentration: 200U/μL
- Unit definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)25 as template-primer
- Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)25, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C
- Optimal reaction temperature: 37°C
- Final product size: 7kb or less
PCR & Real-Time PCR, Reverse Transcription
Order Info
Shipping Condition: Wet ice
Specifications
| 200 U/μL | |
| M-mLV Reverse Transcriptase | |
| Separate components | |
| Reverse Transcription | |
| RNA | |
| 37°C | |
| Yes |
| First-Strand cDNA | |
| RT-PCR, RT-qPCR | |
| 50 min. | |
| Up to 7 kb | |
| Reverse Transcription | |
| M-MLV | |
| Wet Ice |
For Research Use Only. Not for use in diagnostic procedures.