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Invitrogen™ M-MLV Reverse Transcriptase (200 U/µL) DFS Item

Recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid

$486.00 - $2,205.00

Specifications

Concentration 200 U/μL
Final Product Type First-Strand cDNA
Format M-mLV Reverse Transcriptase
PCR Method RT-PCR, RT-qPCR
Reaction Format Separate components
View More Specs

Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity  
Catalog Number Mfr. No. No. of Reactions Price Quantity  
28025013 DFS Item
View Documents Promotion Details
Invitrogen™
28025013
200 Reactions
Each for $486.00
 
28025021 DFS Item
View Documents Promotion Details
Invitrogen™
28025021
1,000 Reactions
Each for $2,205.00
 
Description

Description

Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.
  • Thermostability—optimal activity at 37°C
  • Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7kb
  • Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
  • Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid
  • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
  • Concentration: 200U/μL
  • Unit definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)25 as template-primer
  • Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)25, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Wet ice

Specifications

Specifications

200 U/μL
M-mLV Reverse Transcriptase
Separate components
Reverse Transcription
RNA
37°C
Yes
First-Strand cDNA
RT-PCR, RT-qPCR
50 min.
Up to 7 kb
Reverse Transcription
M-MLV
Wet Ice
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.