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Invitrogen™ ViraPower™ UbC Lentiviral Gateway™ Expression Kit
Facilitates easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and nondividing mammalian cells
Supplier: Invitrogen™ K499000
Includes: pLenti6/UbC/V5-DEST (Box 1): 6μg; pLenti6/UbC/V5-GW/lacZ Control vector (Box 1): 10μg; Stbl3 Chemically Competent E. coli (Box 2): 21 x 50μL; pUC19 control DNA (10pg/μL): 50μL; S.O.C. Medium: 6mL; Support kit (Box 3); ViraPower Packaging Mix (1μg/μL): 195μg; Lipofectamine 2000: 0.75mL; Blasticidin: 50mg; 293 FT cells (3 x 106/mL) (Box 4): 1mL
Description
- Contains all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit
- The pLenti6/UbC/V5-DEST vector has the UbC promoter for driving constitutive but physiological levels of expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells
- Well-suited to long-term experiments
- Accurate titer of functional virus
- Flexible and versatile Gateway recombination cloning technology
- Vector includes UbC promoter and V5 epitope tag at C terminus
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery
Specifications
| Constitutive | |
| UbC | |
| Blasticidin | |
| V5 Epitope Tag | |
| 1 kit | |
| Gateway, ViraPower |
| Lentiviral | |
| Lentiviral Expression Kit | |
| Vector kit (Boxes 1 and 2) pLenti6⁄UbC⁄V5-DEST™ (Box 1): 6 μg, -20°C pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector (Box 1): 10 μg, -20°C One Shot™ Stbl3™ Chemically Competent E. coli (Box 2): 21 x 50 μL, -80°C pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C S.O.C. Medium: 6 mL -80°C Support kit (Box 3) ViraPower™ Packaging Mix (1 μg⁄μL): 195 μg, -20°C Lipofectamine™ 2000: 0.75 mL, +4°C Blasticidin: 50 mg, -20°C 293 FT cells (3 x 106⁄mL) (Box 4): 1 mL, liquid nitrogen |
|
| Gateway | |
| pLenti | |
| Viral Expression |
For Research Use Only. Not for use in diagnostic procedures.