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Invitrogen™ T-REx™ Core Kit, with pcDNA™4/TO Vector 
Regulated mammalian expression system yields exceptionally high levels of induced expression
Supplier: Invitrogen™ K102002
Includes: 20μg of pcDNA4/TO or 20μg each of pcDNA;4/TO/myc-His A, B, and C; a positive control vector, pcDNA;6/TR, and forward and reverse sequencing primers. All vectors are supercoiled and provided lyophilized.
Description
- Uses complete CMV promoter and adds control elements from bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of strongest mammalian promoter sequences known
- Specific Activation: uses a repressor mechanism that blocks transcription from powerful CMV promoter in absence of tetracycline
- Because elements do not use viral transactivators, you can achieve high-level expression from complete CMV promoter without secondary, non-specific activation of host genes
- T-REx transcriptional control elements are provided through two tetracycline operator sequences (TetO2) that have been inserted between the TATA box of the CMV promoter and transcriptional start site
- TetO2 sequence itself has no effect on expression
- Tetracycline repressor protein (TR) is present, it effectively binds TetO2 sites and blocks transcription initiation
- Tetracycline added to culture medium binds to, and changes conformation of, TR protein; Change causes TR protein to release TetO2 sites, derepressing transcription from CMV promoter; The result is high-level expression of the gene of interest
- Expression levels can be modulated based on tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors
- T-REx is powerful inducible mammalian expression system that allows to regulate expression from complete human cytomegalovirus (CMV) enhancer-promoter
- Complete CMV enhancer-promoter sequence containing two copies of tetracycline operator TetO2 sequence for high-level regulated expression
- Zeocinor hygromycin resistance gene for effective selection of stable mammalian cell lines
- Large multiple cloning site to simplify cloning
- In addition, pcDNA™4/TO/myc-His offers a c-myc epitope for rapid detection of recombinant protein with an Anti-myc Antibody and a polyhistidine (6xHis) sequence for simple purification of recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody
- Regulatory vector, pcDNA™6/TR, is provided for high-level expression of tetracycline repressor (TR) protein
- Vector expresses Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express TR protein
Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression
Specifications
| Inducible | |
| Tetracycline | |
| T-Rex Core Cloning Kit with Vector | |
| The T-REx™ Core Kit includes 20 μg of pcDNA™4/TO or 20 μg each of pcDNA™4/TO/myc-His A, B, and C; a positive control vector, pcDNA™6/TR, and forward and reverse sequencing primers. All vectors are supercoiled and provided lyophilized. Store all components at -20°C. All components are guaranteed stable for 6 months when properly stored. |
|
| Restriction Enzyme/MCS | |
| pcDNA | |
| Regulated Expression |
| Transfection | |
| CMV/TO | |
| Zeocin™, Blasticidin | |
| Untagged | |
| 1 kit | |
| Gateway, T-REx, pcDNA |
For Research Use Only. Not for use in diagnostic procedures.