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Invitrogen™ Gateway™ pYES-DEST52 Vector DFS Item

Designed for rapid cloning with Gateway entry clone using lambda phage site-specific recombination and subsequent expression in Saccharomyces cerevisiae

Supplier:  Invitrogen™ 12286019

Catalog No. 12286019


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Includes: The pYES2-DEST52 Gateway destination vector is provided supercoiled and lyophilized.

Description

Description

  • As part of YESVector Collection, pYES2-DEST52 carries promoter and enhancer sequences from GAL1 gene for regulated expression in S.cerevisia
  • 2μ origin of replication for high-copy maintenance
  • Gene for auxotrophic selection on economical minimal medium
  • C-terminal V5 epitope and polyhistidine(6xHis) tags for efficient detection and purification
  • tR sites for efficient recombination with any attL-flanked Gateway entry vector

Cloning, Gateway Cloning, Protein Expression, Proteins, Expression, Isolation and Analysis, S. cerevisiae Expression Systems, Yeast Expression

Specifications

Specifications

GAL1
The pYES2-DEST52 Gateway™ destination vector is provided supercoiled and lyophilized. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.
His Tag (6x), V5 Epitope Tag
6 μg
Gateway
Gateway System Destination Expression Vector
Ampicillin (AmpR)
Gateway
pYES, pDEST
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For Research Use Only. Not for use in diagnostic procedures.