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Invitrogen™ Gateway™ pcDNA™-DEST47 Vector

Designed for high-level, constitutive expression in variety of mammalian cell lines

Supplier: Invitrogen™ 12281010

Catalog No. 12281010

Quantity

Includes: All destination vectors are provided lyophilized and supercoiled.

Requires : Gateway entry clone, appropriate Gateway LR Clonase™ enzyme mix, and reaction buffer.

Description

C-terminal Cycle 3 GFP tag for rapid detection of recombinant protein
Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway cloning, enabling recombination with attL-flanked fragments
Neomycin resistance gene for stable selection
Ampicillin resistance gene and pUC origin for selection and maintenance in E. Coli

Cloning,Constitutive Expression,Gateway Cloning,Mammalian Expression,Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Constitutive or Inducible System	Constitutive
Promoter	CMV
Reporter Gene	GFP (Cycle 3)
Content And Storage	All destination vectors are provided lyophilized and supercoiled.
Cloning Method	Gateway
Vector	pDEST, pcDNA
For Use With (Application)	Constitutive Expression

Delivery Type	Transfection
Product Type	Gateway System Destination Expression Vector
Selection Agent (Eukaryotic)	Geneticin™ (G-418)
Protein Tag	GFP (Cycle 3)
Quantity	6 μg
Product Line	Gateway™, pDEST™

Product Suggestions

For Research Use Only. Not for use in diagnostic procedures.

Description