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Invitrogen™ Gateway™ pcDNA™-DEST40 Vector

Catalog No. 12274015
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Quantity:
6 μg
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Catalog No. Quantity
12274015 6 μg
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Catalog No. 12274015 missing translation for 'mfr' Invitrogen™ missing translation for 'supplierNo' 12274015
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Includes

All destination vectors are provided lyophilized and supercoiled.

Requires

Gateway entry clone, appropriate Gateway LR Clonase™ enzyme mix, and reaction buffer.

Designed for high-level, constitutive expression in variety of mammalian cell lines

  • C-terminal V5-6x His tag for easy detection and rapid purification
  • Cytomegalovirus (CMV) promoter for high-level expression
  • attR sites for Gateway cloning, enabling recombination with attL-flanked fragments
  • Neomycin resistance gene for stable selection
  • Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Cloning,Constitutive Expression,Gateway Cloning,Mammalian Expression,Protein Expression,Proteins, Expression, Isolation and Analysis

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Product Type Gateway System Destination Expression Vector
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage All destination vectors are provided lyophilized and supercoiled.
Protein Tag His Tag (6x), V5 Epitope Tag
Cloning Method Gateway
Quantity 6 μg
Vector pDEST
Product Line Gateway, pDEST
For Use With (Application) Constitutive Expression
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For Research Use Only. Not for use in diagnostic procedures.