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Invitrogen™ pcDNA™6.2-DEST Mammalian Expression Vector

Catalog No. 12489027
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6 μg
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Catalog No. Quantity
12489027 6 μg
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Catalog No. 12489027 Supplier Invitrogen™ Supplier No. 12489027
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Description

Includes

All destination vectors are provided lyophilized and supercoiled.

Requires

Gateway entry clone, Gateway LR Clonase™ enzyme mix, and reaction buffer.

Designed for high-level, constitutive expression in variety of mammalian cell lines

  • Cytomegalovirus (CMV) promoter for high-level expression
  • attR sites for Gateway cloning, enabling recombination with attL-flanked fragments
  • C-terminal V5 tag for easy detection
  • Neomycin resistance gene for stable selection
  • Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
  • Gateway Cloning
  • Destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an UltimateORF Clone

Cloning,Constitutive Expression,Gateway Cloning,Mammalian Expression,Protein Expression,Proteins, Expression, Isolation and Analysis

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Product Type Mammalian Expression Vector
Selection Agent (Eukaryotic) Blasticidin
Content And Storage All destination vectors are provided lyophilized and supercoiled.
Protein Tag V5 Epitope Tag
Cloning Method Gateway
Quantity 6 μg
Vector pDEST, pcDNA
Product Line Gateway, pcDNA
For Use With (Application) Constitutive Expression
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For Research Use Only. Not for use in diagnostic procedures.