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Invitrogen™ pcDNA™3.2-DEST Mammalian Expression Vector 
Designed for high-level, constitutive expression in variety of mammalian cell lines
Supplier: Invitrogen™ 12489019
Comprend: All destination vectors are provided lyophilized and supercoiled.
Exige : Gateway entry clone, Gateway LR Clonase™ enzyme mix, and reaction buffer.
Description
- Cytomegalovirus (CMV) promoter for high-level expression
- attR sites for Gateway cloning, enabling recombination with attL-flanked fragments
- C-terminal V5 tag for easy detection
- Neomycin resistance gene for stable selection
- Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
- Gateway Cloning
Antibody and Cell Culture Production,Bioproduction,Cloning,Constitutive Expression,Gateway Cloning,Mammalian Expression.Protein Expression,Proteins, Expression, Isolation and Analysis
Spécifications
| Constitutive | |
| CMV | |
| Geneticin™ (G-418) | |
| V5 Epitope Tag | |
| 6 μg | |
| Gateway™, pcDNA™ |
| Transfection | |
| Mammalian Expression Vector | |
| All destination vectors are provided lyophilized and supercoiled. | |
| Gateway | |
| pDEST, pcDNA | |
| Constitutive Expression |
For Research Use Only. Not for use in diagnostic procedures.