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Invitrogen™ pAd/CMV/V5-DEST™ Gateway™ Vector Kit
For easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and nondividing mammalian cells
Supplier: Invitrogen™ V49320
Includes: pAd-CMV/V5-DEST Vector 150ng/μL in TEBuffer, pH 8.0. 40μL; pAd/CMV/V5-GW/lacZ control plasmid 1μg/μL in TE Buffer, pH 8.0, 10μL.
Description
- The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter
- High efficiency and rapid recombination cloning
- Produces high titer adenoviral stocks
- Efficient delivery of the gene to dividing and nondividing mammalian cells in vitro or in vivo
- Produces replication-incompetent virus for enhanced biosafety of the system
- Amenable for use in high-throughput applications
- CMV promoter for high-level constitutive expression of gene of interest
- Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
- Herpes Simplex Virus thymidine kinase (TK) polyadenylation sequence for efficient transcription termination and polyadenylation of mRNA
- V5 epitope for detection of recombinant protein
- Ampicillin selection marker
Antibody and Cell Culture Production, Bioproduction, Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery, Viral Vaccine Production
Specifications
| Constitutive | |
| CMV | |
| • pAd-CMV⁄V5-DEST™ Vector 150 ng⁄μl in TEBuffer, pH 8.0. 40 μl (Store at -20°C) • pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 μg⁄μl in TE Buffer, pH 8.0. 10 μl (Store at -20°C) |
|
| Gateway | |
| pDEST | |
| Viral Expression |
| Adenoviral | |
| Mammalian Expression Vector | |
| V5 Epitope Tag | |
| 6 μg | |
| Gateway, ViraPower |
For Research Use Only. Not for use in diagnostic procedures.