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Invitrogen™ Baculovirus Expression System with Gateway™ Technology 
Designed to create recombinant pFastBac™ plasmids containing polyhedrin promoter
Supplier: Invitrogen™ 11827011
Includes: LR Clonase enzyme mix and reaction buffer, destination vectors pDEST8, pDEST10, and pDEST20, proteinase K solution (2μg/μL), pENTR™-gus positive control, and Library Efficiency DH5Competent Cells. The kit does not include MAX Efficiency DH10Bac Competent Cells.
Description
- Destination vectors carrying polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins
- pDEST™10 vector containing TEV protease cleavage site for removal of fusion tag after protein purification
Baculovirus Production and Titering/Insect Expression/Protein Expression/Proteins, Expression, Isolation and Analysis
Specifications
| Polyhedrin | |
| The Baculovirus Expression System with Gateway™ Technology includes LR Clonase™ enzyme mix and reaction buffer, destination vectors pDEST™8, pDEST™10, and pDEST™20, proteinase K solution (2 μg/μl), pENTR™-gus positive control, and Library Efficiency™ DH5Competent Cells. The kit does not include MAX Efficiency™ DH10Bac™ Competent Cells. Store LR Clonase™ enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored. | |
| Gateway | |
| pDEST, Gateway Baculovirus Vectors |
| Expression System | |
| GST Tag, His Tag (6x), Untagged | |
| 20 reactions | |
| Gateway |
For Research Use Only. Not for use in diagnostic procedures.